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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
PDGF BB protein (Active)
See all PDGF BB products (9) ...
Protein description
Recombinant Full Length human PDGF BB is a homodimeric, glycosylated, polypeptide chain containing 109 (X 2) amino acids and having a molecular mass of 32 kDa, purified from Saccharomyces cerevisiae. (SwissProt P01127).
Properties
Purity
>95% by SDS-PAGE
Purification notes
This protein was purified by proprietary chromatographic techniques. Purity greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological activity
Specific Activity of 1 MIU/mg.
Form
Lyophilised:
Reconstitute the protein in sterile 18Mohm/cm H2O at not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 20mM Sodium acetate
Concentration
Concentration information loading...
Sequence notes
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gl y-Ser-Leu.
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Human
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE
Functional Studies
Application notes
Biological Activity: The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Target information above from: UniProt accession
P01127
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for PDGF BB protein (Active) (ab73229)
This product has been referenced in:
- Hou WHet al. CRSBP-1/LYVE-1 ligands disrupt lymphatic intercellular adhesion by inducing tyrosine phosphorylation and internalization of VE-cadherin. J Cell Sci 124:1231-44 (2011). Mouse.Read more (PubMed: 21444752) »
See 1 publication for this product
Publishing research using ab73229? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"