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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
PDGF BB protein (Active)
See all PDGF BB products (9) ...
Protein description
Recombinant Full Length Mouse PDGF BB protein, 109 amino acids. MW 25 kDa. Swiss ID = P31240.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Purification notes
ab73233 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
Biological activity
Biological Activity: The ED50 range= 1-5 ng/ml, determined by the dose-dependant proliferation of BALB/c 3T3.
Form
Lyophilised:
Reconstitute in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab73233 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Storage instructions
Lyophilized protein should be stored below -20°C. Upon reconstitution aliquot and store below -18°C to prevent freeze-thaw cycles.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage buffer
Preservative: None
Concentration
Concentration information loading...
Sequence notes
The sequence of the first five N-terminal amino acids was determined to be Ser-Leu-Gl y-Ser-Leu.
Research areas
Stem Cells >> Stem Cell Cytokines >> Recombinant Mouse
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
SDS-PAGE
Application notes
FuncS: Dose-dependant proliferation of BALB/c 3T3. Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Target information above from: UniProt accessionP01127
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for PDGF BB protein (Active) (ab73233)
ab73233 has not yet been referenced specifically in any publications.
Publishing research using ab73233? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"