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Product Name
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PDGF BB protein (Active)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant Full Length Mouse PDGF BB protein, 109 amino acids.
MW 25 kDa. Swiss ID = P31240.
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Specificity
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Biological Activity:
The ED50 range= 1-5 ng/ml, determined by the dose-dependant proliferation of BALB/c 3T3.
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Tested applications
(see key)
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FuncS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
FuncS: Dose-dependant proliferation of BALB/c 3T3. Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Stem Cells >> Stem Cell Cytokines >> Recombinant Mouse
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 18591 (Mouse)
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
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Purification notes
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ab73233 is purified by proprietary chromatographic techniques.
Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
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Form
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Lyophilised
Reconstitute in sterile 100mM acetic acid and 0.1% BSA not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Upon reconstitution ab73233 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Storage instructions
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The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.
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Expression system
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E. coli
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Sequence
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The sequence of the first five N-terminal amino acids was determined to be Ser-Leu-Gly-Ser-Leu.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDGF BB protein (Active) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to PDGF BB
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