PDGF BB protein (Active) (ab9706)

Hi,

Thanks for that, I just have one remaining question about its

reconstitution, by carrier protein do you mean Human or bovine serum

albumin as stated for the reconstition of ab51925? Help on this would

be most useful

ANSWER:

Yes, either HSA or BSA will be fine to use in the reconstitution of ab9706. We would also recommend a quick spin in a microcentrifuge prior to opening the vial to make sure the protein gets down to the bottom of the vial, then using sterile H2O and adding a carrier protein as mentioned if diluting below 0.1mg/ml (for example 0.1% BSA). If you have any further questions please do not hesitate to ask.

Hi,

I am a researcher at Edinburgh University and I have just ordered

and received PDGF BB protein (ab9706) for use in growth medium

Purchase Order Number 4506313210/50F. Upon looking for its

reconstitution I have discovered another PDGF BB protein (ab51925)

with the abbreviation (Active). Can PDGF BB protein ab9706 be used for

my purpose or is it inactive? If so I can return the Inactive form and

have it replaced with the active form as the inactive form is of no

use for these purposes. I think that if this is inactive, it should

have been made clear on the website otherwise I would clearly not have

purchased this. I wish to commence a series of very expensive

experiments which will require continued use of this growth factor,

could you please clarify this as soon as possible,

ANSWER:

Thank you for contacting us. Both PDGF BB protein ab9706 (human) and ab51925 (mouse) have been assessed for activity by dose-dependent stimulation of the proliferation of Balb/c 3T3 cells with ED50 of 1.0-3.0 ng/ml and 2 ng/ml resepectively. They are both active. I am sorry that the information provided on the website has been confusing and thank you for bringing this to our attention. I will be amending this for future customers.

ab9706 can be reconstitution with water, however a carrier protein is required if aliquotting under 0.1mg/ml. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

How should this product be reconstituted?

ANSWER:

Thank you for your enquiry.

Human PDGF-BB, ab9706, is reconstituted in water:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

Please do not hesitate to contact me should you have any additional questions.

What are the reconstitution instructions for this protein? I was also wondering if I need to centrifuge it before opening it. I apperciate your help in advance. Thanks,

ANSWER:

Thank you for your enquiry.

Yes, we would highly recommend centrifuging the vial before opening, as some of the contents may have attached to the cap region during transport. To reconstitute this product, you can dilute in water. The volume of water depends on the concentration of the product itself, which is 10ug. Then, you can dilute the product in any other buffer in order to place it in working aliquots.

I hope this information will be useful. Should you require any further assistance, please do not hesitate to contact me.

Can PDGF-BB be diluted in water? If not, what can I dilute it in?

ANSWER:

Thank you for your enquiry. Yes, this product can be diluted water. The volume of water depends on the concentration of the product itself. Then, you can dilute the product in any other buffer in order to place it in working aliquots. Have you purchased this product? If so, could you please reply with the lot number which is on the vial label?