Biotin Anti-PDGFR alpha antibody [APA5] (ab93530)
Key features and details
- Biotin Rat monoclonal [APA5] to PDGFR alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-PDGFR alpha antibody [APA5]
See all PDGFR alpha primary antibodies -
Description
Biotin Rat monoclonal [APA5] to PDGFR alpha -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse PDGFR alpha. Extracellular domain of PDGFRa and the Fc portion of human IgG1
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Positive control
- NIH/3T3 cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
APA5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.5-1µg for 105-8 cells.
A final volume of 100 µl is recommended for staining the cell sample.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 0.5-1µg for 105-8 cells. A final volume of 100 µl is recommended for staining the cell sample.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Function
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18595 Mouse
- SwissProt: P26618 Mouse
- Unigene: 221403 Mouse
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Alternative names
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab93530 has been referenced in 2 publications.
- Miyawaki T et al. Tlx, an orphan nuclear receptor, regulates cell numbers and astrocyte development in the developing retina. J Neurosci 24:8124-34 (2004). IHC-Fr . PubMed: 15371513
- Takakura N et al. PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5. J Histochem Cytochem 45:883-93 (1997). IHC-P . PubMed: 9199674