Biotin Anti-PDGFR alpha antibody [16A1] (ab99924)
Key features and details
- Biotin Mouse monoclonal [16A1] to PDGFR alpha
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-PDGFR alpha antibody [16A1]
See all PDGFR alpha primary antibodies -
Description
Biotin Mouse monoclonal [16A1] to PDGFR alpha -
Host species
Mouse -
Conjugation
Biotin -
Specificity
recognizes CD140a / PDGF receptor alpha, the 170 kDa alpha chain of platelet derived growth factor receptor, which is widely expressed on a variety of mesenchymal derived cells and plays pro proliferative or anti proliferative roles in various tumours. -
Tested applications
Suitable for: ICC, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human PDGFR alpha. CD140a-transfected NIH 3T3 cells
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General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
Purified by immunoaffinity chromatography (> 95% by SDS-PAGE) prior to conjugation. -
Clonality
Monoclonal -
Clone number
16A1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5156 Human
- Omim: 173490 Human
- SwissProt: P16234 Human
- Unigene: 74615 Human
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Alternative names
- Alpha-type platelet-derived growth factor receptor antibody
- CD140 antigen-like family member A antibody
- CD140a antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99924 has not yet been referenced specifically in any publications.