Anti-PDGF Receptor alpha antibody (ab124392)

Overview

  • Product nameAnti-PDGF Receptor alpha antibody
    See all PDGF Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to PDGF Receptor alpha
  • Tested applicationsWB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:C-

    GKRNRHSSQTSEESAIETG

    conjugated to KLH, corresponding to amino acids 1035-1053 of Human PDGF Receptor alpha.

  • Positive control
    • Human brain, cerebellum tissue; NIH/3T3 cell line lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Detects a band of approximately 150,170 kDa (predicted molecular weight: 122 kDa).
IP Use at an assay dependent concentration. 5-10 µl immunoprecipitates PDGF Receptor alpha from 500 µg of NIH/3T3 RIPA lysate.
IHC-P 1/100.

Target

  • FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
  • Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-type platelet-derived growth factor receptor antibody
    • CD140 antigen-like family member A antibody
    • CD140a antibody
    • CD140a antigen antibody
    • MGC74795 antibody
    • PDGF alpha chain antibody
    • PDGF-R-alpha antibody
    • PDGFR 2 antibody
    • PDGFR alpha antibody
    • PDGFR2 antibody
    • PDGFRA antibody
    • PDGFRA/BCR fusion antibody
    • PGFRA_HUMAN antibody
    • Platelet derived growth factor receptor 2 antibody
    • Platelet derived growth factor receptor alpha antibody
    • Platelet derived growth factor receptor alpha polypeptide antibody
    • Platelet derived growth factor receptor antibody
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibody
    • RHEPDGFRA antibody
    see all

Anti-PDGF Receptor alpha antibody images

  • ab124392, at 1/100 dilution, staining PDGF Receptor alpha in formalin-fixed, paraffin-embedded Human brain cerebellum tissue by Immunohistochemistry.

References for Anti-PDGF Receptor alpha antibody (ab124392)

ab124392 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization No
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formalin-fixed paraffin-embedded sections
Username

Abcam user community

Verified customer

Submitted Aug 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"