Anti-PDGF Receptor alpha antibody (ab65258)

Overview

• Product nameAnti-PDGF Receptor alpha antibodySee all PDGF Receptor alpha primary antibodies ...
• Description
  Rabbit polyclonal to PDGF Receptor alpha
• SpecificityThis antibody will cross-react with both the phosphorylated and non-phosphorylated protein.
• Tested applicationsELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y720 of human PDGF Receptor alpha.

• Positive control293 cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was purified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Growth factors
  • PDGF

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • PDGF

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • Other

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

Applications

Target

• FunctionReceptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
• Tissue specificityExpressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.
• Involvement in diseaseNote=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
  Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession P16234 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5156 Human
  • Entrez Gene: 18595 Mouse
  • Omim: 173490 Human
  • SwissProt: P16234 Human
  • SwissProt: P26618 Mouse
  • Unigene: 74615 Human
  • Unigene: 221403 Mouse

• Alternative names
  Alpha platelet derived growth factor receptor antibodyAlpha-type platelet-derived growth factor receptor antibodyCD 140a antibody

  CD140 antigen-like family member A antibodyCD140a antibodyCD140a antigen antibodyMGC74795 antibodyPDGF alpha chain antibodyPDGF R alpha antibodyPDGF-R-alpha antibodyPDGFR 2 antibodyPDGFR A antibodyPDGFR alpha antibodyPDGFR2 antibodyPDGFRA antibodyPDGFRA/BCR fusion antibodyPGFRA_HUMAN antibodyPlatelet derived growth factor receptor 2 antibodyPlatelet derived growth factor receptor alpha antibodyPlatelet derived growth factor receptor alpha polypeptide antibodyPlatelet derived growth factor receptor antibodyRearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein antibodyRHEPDGFRA antibody

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Anti-PDGF Receptor alpha antibody images

• 

  Western blot - PDGF Receptor alpha antibody (ab65258)
  All lanes : Anti-PDGF Receptor alpha antibody (ab65258) at 1/50 dilution
  
  Lane 1 : 293 cell lysate, nontransfected
  Lane 2 : 293 cell lysate, transiently transfected with the PDGFRA gene
  
  Lysates/proteins at 2 µg per lane.
  
  
  Predicted band size : 123 kDa
  Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.