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PDGF Receptor beta Human ELISA Kit (ab100626)

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Overview

  • Product namePDGF Receptor beta Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 25 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90.39 76% - 104%
    Serum 109.6 97% - 118%
    Plasma 96.23 81% - 117%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100626 Human PDGF Receptor beta (platelet derived growth factor receptor beta; PDGFR-beta) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF Receptor beta in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human PDGF Receptor beta coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF Receptor beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PDGF Receptor beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF Receptor beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionGrowth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • Tissue specificityExpressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Cellular localizationSecreted. Released by platelets upon wounding.

    • Target information above from: UniProt accession P01127 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Beta type platelet derived growth factor receptorCD140 antigen like family member BCD140b
        JTK12PDGF R betaPDGF subunit BPDGF-2PdgfbPDGFB_HUMANPDGFRPDGFR betaPDGFR1PDGFRBPlatelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptidePlatelet-derived growth factor B chainPlatelet-derived growth factor beta polypeptidePlatelet-derived growth factor subunit BProto-oncogene c-Sis
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100626 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      PDGF Receptor beta Human ELISA Kit images

      • ELISA - PDGF Receptor beta Human ELISA Kit (ab100626)
        ELISA - PDGF Receptor beta Human ELISA Kit (ab100626)

      Protocols

      References for PDGF Receptor beta Human ELISA Kit (ab100626)

      ab100626 has not yet been referenced specifically in any publications.

      Publishing research using ab100626 ? Please let us know so that we can cite the reference in this datasheet

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