Anti-PDGF Receptor beta (phospho Y740) antibody (ab16863)

Overview

• Product nameAnti-PDGF Receptor beta (phospho Y740) antibodySee all PDGF Receptor beta primary antibodies ...
• Description
  Rabbit polyclonal to PDGF Receptor beta (phospho Y740)
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH corresponding to residues surrounding Tyr740 of human PDGF Receptor beta

• Positive controlNIH/3T3 cell lysates stimulated with PDGF-BB

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Growth factors
  • PDGF

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • PDGF

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • Other

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

Applications

Target

• FunctionReceptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
• Involvement in diseaseNote=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
  Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
  Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
  Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
  Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
  Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
• Cellular localizationMembrane.

Target information above from: UniProt accession P09619 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5159 Human
  • Omim: 173410 Human
  • SwissProt: P09619 Human
  • Unigene: 509067 Human

• Alternative names
  Beta platelet derived growth factor receptor antibodyBeta-type platelet-derived growth factor receptor antibodyCD 140B antibody

  CD140 antigen-like family member B antibodyCD140B antibodyCD140B antibodyCD140b antigen antibodyIBGC4 antibodyJTK12 antibodyOTTHUMP00000160528 antibodyPDGF R beta antibodyPDGF-R-beta antibodyPDGFR 1 antibodyPDGFR antibodyPDGFR beta antibodyPDGFR1 antibodyPDGFRB antibodyPGFRB_HUMAN antibodyPlatelet derived growth factor receptor 1 antibodyPlatelet derived growth factor receptor beta antibodyPlatelet derived growth factor receptor beta polypeptide antibody

  see all

Anti-PDGF Receptor beta (phospho Y740) antibody images

• 

  Western blot - PDGF Receptor beta (phospho Y740) antibody (ab16863)
  
  
  Predicted band size : 124 kDa
  
  
  Western blot analysis of ab16863 in cell lysate: HepG2 cells were transfected with wild-type or Y740F mutants of PDGF receptor beta .PDGFR beta transfected HepG2 cells were either untreated (-) or treated with PDGF-BB (+). The lower panel shows western blotting with unphosphorylated PDGF Receptor beta antibody.