Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Kits
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
PDH E1 alpha protein (PDHA1) ELISA Kit
See all Pyruvate Dehydrogenase E1-alpha subunit products (4) ...
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | = 2.3% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 4 | = 8.2% |
Tests
1 x 96 test
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Quantitative
Sensitivity
= 15 ng/ml
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
ab115342 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of PDHA1 protein in cell and tissue lysates. The assay employs an antibody specific for PDHA1 protein coated on a 96-well plate. Standards and samples are pipetted into the wells and PDHA1 protein present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-PDHA1 protein detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of PDHA1 protein bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Store all components at 4°C. This kit is stable for at least 6 months
from receipt. After reconstitution the standard should be stored at -
80°C. Unused microplate strips should be returned to the pouch
containing the desiccant and resealed.
from receipt. After reconstitution the standard should be stored at -
80°C. Unused microplate strips should be returned to the pouch
containing the desiccant and resealed.
Tested applications
ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 10X PDHA1 protein Detector Antibody | 1 x 1ml | |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| Human PDHA1 protein standard (1 ug) | 1 vial | |
| Microplate 96 antibody coated wells in 12 strips | 1 unit | |
| TMB Development Solution | P/N 8209803 | 1 x 12ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Dehydrogenase Kits
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Alternative names
- MS964
Applications
Show applications key
Our Abpromise guarantee covers the use of ab115342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
PDH E1 alpha protein (PDHA1) ELISA Kit images:
ELISA - PDH E1 alpha protein (PDHA1) ELISA Kit (ab115342)
Example of standard curve constructed using ab115342.
ELISA - PDH E1 alpha protein (PDHA1) ELISA Kit (ab115342)
Example HeLa dilution series and working range produced with ab115342.
ELISA - PDH E1 alpha protein (PDHA1) ELISA Kit (ab115342)
An experiment to determine the levels of PDHA1 in various fibroblast cell lines. A fibroblast cell line (P) harboring a mutation in the X-chromosome PDHA1 gene results in significant reduction in E1a protein expression. This is compared to control adult fibroblast cells (control) and mouse fibroblast cells (NIH3T3 and MEF, mouse embryonic fibroblasts).
Protocols
References for PDH E1 alpha protein (PDHA1) ELISA Kit (ab115342)
ab115342 has not yet been referenced specifically in any publications.
Publishing research using ab115342? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"