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Overview

  • Product namePDH Immunocapture KitSee all Pyruvate dehydrogenase kits ...
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of PDH complex from heart mitochondria.

    Note: The immunocapture protocol for this kit requires Abcam reagent detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIP more details

Properties

  • Alternative names
      PDCPDHPDHC

Applications

Our Abpromise guarantee covers the use of ab109802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP

PDH Immunocapture Kit images

  • Immunoprecipitation - PDH Immunocapture Kit (ab109802)
    Immunoprecipitation - PDH Immunocapture Kit (ab109802)
    Complex I immunoprecipitation using antibody ab109866/MS101c crosslinked to protein G-agarose beads as product ab109802

Protocols

References for PDH Immunocapture Kit (ab109802)

This product has been referenced in:
  • Stenson BM  et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). Read more (PubMed: 19556420) »
  • Bonnet S  et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 11:37-51 (2007). Read more (PubMed: 17222789) »

See all 2 Publications for this product

Publishing research using ab109802 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab109802 Submit a Question for ab109802

Displaying 1 - 6 of 6 results for Abreviews and Q&A

10
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How many tests can be performed with each size kit?  Can you please send me a quote (in GBP) for this kit and for an antibody to detect the E1 alpha subunit?

Thank you for contacting us. 
This kit size refers to the amount of antibody.  For example, the 250 ug kit contains 250 ug of antibody conjugated to 25 uL of beads (settled volume) suspended in a total volume of 400 uL. Thus the concentration of the ...

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I have a customer interested in the following product:

# MSP01-500 (ab109802) Pyruvate Dehydrogenase Complex (PDH) Immunocapture kit

Can you give evidence to prove this immunocapture works. She is planning to apply this kit in mouse tiss...

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Thank you for contacting us.

We haven't tested this kit with mouse samples so we unfortunately cannot provide any details. The kit has been optimized with cow and human samples so these samples are fully guaranteed.

We however recommend ...

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Inquiry: Hi, I am interested in trying this product with rat samples. Do you have any direct evidence for its efficacy against rat PDH, do you know if it was raised against a sequence that is identical to rat? The product information states that it reacts...

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Thank you for contacting us.

I have had the opportunity to speak with the lab regarding this on your behalf. They recommend using ab110333 (MSP06) with rat samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH comp...

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Hello,
I have been already helped because I sent her a separate email amended to my previous inquiry from half a year ago. I also got another reference number yesterday from mailto:uk.technical@abcam.com which is different from the one you just gave ...

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Thank you for your previous email and I apologize that I'm just now getting around to reading it! I'll close any other correspondance so you won't be bothered again about this enquiry.

If you need any further information about these products ple...

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Hello again,
I had contacted you with a question concerning your PDH-capture antibody (ab109802 or MSP01) about half a year ago, and got a very good answer, so I was wondering if I could ask one more thing. I realize that it most likely is not possib...

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Thank you for contacting us with your question, and for your patience while I have been in touch with the lab about the specificity of this antibody.
My contact at the lab has responded:
"We did mass spectrometry and immunoblotting using th...

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Hello, I'm interested in your PDH capture antibody (MSP01) but I was wondering if you could tell me, whether the antibody captures the whole complex along with the kinases and phosphatases. So not just E1a, E1b, E2, E3, E3BP but also the PDKs (PDK1,2,...

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Thank you for contacting us and for your interest in MSP01 (ab109802). I forwarded your email to the lab scientists at MitoSciences, and I received the following response: We do not know if the kinase and phosphatase are present, but if so...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"