Overview

  • Product namePDH Immunocapture KitSee all Pyruvate dehydrogenase kits ...
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    250 µg, 500 µg or 750 µg monoclonal antibody irreversibly crosslinked to protein G-agarose beads which can immunocapture ~13 µg, ~26 µg or ~39 µg respectively of PDH complex from heart mitochondria.

    Note: The immunocapture protocol for this kit requires Abcam reagent detergent lauryl maltoside (ab109857/MS910).

  • Tested applicationsIP more details

Properties

  • Alternative names
    • PDC
    • PDH
    • PDHC

Applications

Our Abpromise guarantee covers the use of ab109802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP Use at an assay dependent dilution.

PDH Immunocapture Kit images

  • PDH immunoprecipitation using antibody ab109866/MSP01c crosslinked to protein G-agarose beads as product ab109802

Protocols

References for PDH Immunocapture Kit (ab109802)

This product has been referenced in:
  • Stenson BM  et al. Activation of liver X receptor regulates substrate oxidation in white adipocytes. Endocrinology 150:4104-13 (2009). Read more (PubMed: 19556420) »
  • Bonnet S  et al. A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell 11:37-51 (2007). Read more (PubMed: 17222789) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. 
This kit size refers to the amount of antibody.  For example, the 250 ug kit contains 250 ug of antibody conjugated to 25 uL of beads (settled volume) suspended in a total volume of 400 uL. Thus the concentration of t...

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Thank you for contacting us.

We haven't tested this kit with mouse samples so we unfortunately cannot provide any details. The kit has been optimized with cow and human samples so these samples are fully guaranteed.

We however recomme...

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I have had the opportunity to speak with the lab regarding this on your behalf. They recommend using ab110333 (MSP06) with rat samples. The antibody targets on E2/E3 binding protein but pulls down the whole PDH c...

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Thank you for your previous email and I apologize that I'm just now getting around to reading it! I'll close any other correspondance so you won't be bothered again about this enquiry.

If you need any further information about these products ...

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Thank you for contacting us with your question, and for your patience while I have been in touch with the lab about the specificity of this antibody.
My contact at the lab has responded:
"We did mass spectrometry and immunoblotting using...

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Thank you for contacting us and for your interest in MSP01 (ab109802). I forwarded your email to the lab scientists at MitoSciences, and I received the following response: We do not know if the kinase and phosphatase are present, but if...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"