Recombinant Anti-PDIA2/PDIP antibody [EPR12212] (ab180931)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12212] to PDIA2/PDIP
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PDIA2/PDIP antibody [EPR12212]
See all PDIA2/PDIP primary antibodies -
Description
Rabbit monoclonal [EPR12212] to PDIA2/PDIP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human pancreas, HeLa, BxPC-3 and HepG2 cell lysates; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12212 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
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Notes |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. -
Tissue specificity
Highly expressed in pancreas (at protein level). -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Post-translational
modificationsThe disulfide-linked homodimer exhibits an enhanced chaperone activity.
Glycosylated. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 64714 Human
- Entrez Gene: 69191 Mouse
- Entrez Gene: 287164 Rat
- Omim: 608012 Human
- SwissProt: Q13087 Human
- SwissProt: D3Z6P0 Mouse
- SwissProt: P04785 Rat
- Unigene: 66581 Human
see all -
Alternative names
- Pancreas specific protein disulfide isomerase antibody
- Pancreas-specific protein disulfide isomerase antibody
- Pancreatic protein disulfide isomerase antibody
see all
Images
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Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed)labeling PDIA2/PDIP with ab180931 at 1/100 dilution (green) or Rabbit monoclonal IgG (red), followed with Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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All lanes : Anti-PDIA2/PDIP antibody [EPR12212] (ab180931) at 1/20000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 58 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180931 has not yet been referenced specifically in any publications.