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Western blot - PDI antibody - ER Marker (ab3672)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDI antibody - ER Marker (ab3672)
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Immunohistochemistry (Frozen sections) - PDI antibody - ER Marker (ab3672)
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Product Name
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PDI antibody - ER Marker
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See all PDI - ER marker antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PDI - ER Marker
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Immunogen
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Full length protein.
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Reacts with
(species key)
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Hu, Ms, Rat, AGMk, Cow, Hm
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Specificity
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PDI runs at 61.2 kD on samples from human placenta. In samples from rat liver, it runs at 59 kD, but 3 other bands may appear due to protein degradation - these run at 58.9 kD, 55.2 kD and 53.8 kD.
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Tested applications
(see key)
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/50 - 1/100.
ICC/IF: 1/600 (PMID 19059915).
IHC-Fr: 1/100.
WB: 1/1000. Detects a band of approximately ~60 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Endoplasmic reticulum lumen.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
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Relevance
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The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.1% Sodium Azide
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDI antibody - ER Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PDI antibody - ER Marker images, references, products related to ab3672 and other tools.
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PDI antibody - ER Marker images:
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 Western blot - PDI antibody - ER Marker (ab3672)
PDI antibody - ER Marker (ab3672) at 1/1000 dilution + PC12 whole cell lysate at 20 µg
Secondary
HRP conjugated swine anti-rabbit antibody
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,48 kDa,58 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDI antibody - ER Marker (ab3672)
ab3672 at 1/400 staining mouse kidney tissue sections by IHC-P. The tissue was formladehyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer. The tissue was incubated with the primary antibody for 30 minutes at 22°C and then an HRP conjugated goat anti-rabbit antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Frozen sections) - PDI antibody - ER Marker (ab3672)
ab3672 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr. Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours at 25°C. A TRITC conjugated pig anti-rabbit IgG antibody, diluted 1/1000, was used as the secondary. This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrus
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDI antibody - ER Marker (ab3672)
ab3672 staining PDI - ER in human HaCaT Keratinocyte by ICC/IF. The cells were paraformaldehyde fixed, permeabilized in 0.25% triton X-100 buffer and blocked in 2.5% BSA plus 1% goat serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with sample for 1 hour at 24°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence-PDI antibody - ER Marker(ab3672)
ICC/IF image of ab3672 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3672, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
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References for PDI antibody - ER Marker (ab3672)
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This product has been used in: (two most recent references)
Juanes ME et al. Biosynthesis of prion protein nucleocytoplasmic isoforms by alternative initiation of translation. J Biol Chem 284:2787-94 (2009). WB, ICC/IF; Chinese Hamster. PubMed: 19059915
Lisse TS et al. ER stress-mediated apoptosis in a new mouse model of osteogenesis imperfecta. PLoS Genet 4:e7 (2008). PubMed: 18248096
See all 5 publication references for this product.
If you publish research using ab3672 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PDI
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