Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER
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I would like to take information regarding PDI antibody. There is no mention about concentration for your product nr ab5484. There is only written volume. May be there is written anywhere but I miss it anyway. Please send mail as soon as possible and write concentration. |
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ANSWER: |
Thank you for your enquiry. We are sorry to inform you that this antibody is provided as ascitic fluid and not affinity purified product; therefore the IgG concentration has not been determined. |
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a customer wants to know if this antibody can be used on yeast samples. Thank you in advance for your help.
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ANSWER: |
Thanks for your message. All the information that we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with yeast has not yet been tested. Ab5484 has only been tested for cross-reactivity with Hamster, Human, Mouse and Rat. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunolocalization of PDI in rat epithelial cells using ab5484.
IHC image of ab5484 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5484, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing HeLa cells stained with ab5484 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5484, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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