Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)

Ab2792 positively staining dog MDCK cell ER (red) at 1/200. Staining was carried out in conjunction with goat anti mouse H and L ( Alexa 546) at 1/1000. The nuclei can be seen stained with Hoechst (blue)

This image is courtesy of an Abreview submitted by Kun Liu on 20 September 2005. We do not have any further information relating to this image.

Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)

Ab2792 positively staining formaldehyde fixed human Hek293 cells (1/200). This Ab was used in conjunction with goat anti mouse Alexa Flour ® 546 (1/1500).

The nuclei has been atained with Hoechst.

This image is courtesy of an Abreview submitted by Kun Liu on 19th September 2005. We do not have any further information relating to this image.

Western blot - PDI antibody [RL90] - ER Marker (ab2792)

Predicted band size : 58 kDa

Immunocytochemistry/ Immunofluorescence - PDI antibody [RL90] - ER Marker (ab2792)

ab2792 staining PDI from human HaCaT keratinocyte cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.25 % Triton ×100 and blocking with 2.5% BSA plus 1% goat serum for 1 hour at 4⁰C was performed. Samples were incubated with primary antibody, diluted 1/100, for 1 hour at 24⁰C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/1000 as secondary antibody.

This image is a courtesy of Anonymous Abreview

Western blot - PDI antibody [RL90] - ER Marker (ab2792)

Anti-PDI antibody [RL90] - ER Marker (ab2792) at 1/2000 dilution

Secondary
Donkey anti mouse IgG2a at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 58 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)


Exposure time : 20 seconds

This image is courtesy of an anonymous Abreview