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Anti-PDI antibody [RL90] - ER Marker (ab2792)

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Overview

Product name

Anti-PDI antibody [RL90] - ER Marker
See all PDI products (10) ...

Description

Mouse monoclonal [RL90] to PDI - ER Marker

Tested applications

ICC/IF, Electron Microscopy, IHC-P, IHC-Fr, IP, WBmore details

Cross reactivity

Reacts with

Rat, Hamster, Dog, Human, Monkey

Predicted to work with

Mouse, Fruit fly (Drosophila melanogaster)

Immunogen

Full length native protein (purified) (Rat).

Positive control

rat liver

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

RL90

Isotype

IgG2a

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER

  • Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792) image (enlarge)

  • Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792) image (enlarge)

  • Western blot - PDI antibody [RL90] - ER Marker (ab2792)Western blot - PDI antibody [RL90] - ER Marker (ab2792) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide12 Images

    ICC/IF

    Four stars (14 Abreviews) ICC/IF: 1/100(PubMed: 17...Read more →

    ICC/IF: 1/100(PubMed: 17308099)

  • ShowHide

    Electron Microscopy

     EM: Use at an assay depe...Read more →

    EM: Use at an assay dependent concentration. (PubMed: 21886772)

  • ShowHide

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

  • ShowHide

    IHC-Fr

    Two stars (1 Abreview) IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution. (This antibody has been shown to inhibit the activity of PDI in vitro. It has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.)

  • ShowHide10 Images

    WB

    Five stars (9 Abreviews) WB: 1/1000Detects a band...Read more →

    WB: 1/1000Detects a band of approximately 59-61 kDa (predicted molecular weight: 58 kDa).(If there is no signal or signal is weak, more concentrated antibody could be used in addition to using less stringent blocking conditions (e.g., BSA instead of milk, incubating the antibody in PBST or TBST only, lower milk percentage).)

Target

Function

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.

Tissue specificity

Highly expressed in pancreas (at protein level).

Sequence similarities

Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains.

Post-translational
modifications

The disulfide-linked homodimer exhibits an enhanced chaperone activity.
Glycosylated.

Cellular localization

Endoplasmic reticulum lumen.

Target information above from: UniProt accessionQ13087 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • P4HB antibody
  • Pancreas specific protein disulfide isomerase antibody
  • Pancreas-specific protein disulfide isomerase antibody
  • Pancreatic protein disulfide isomerase antibody
  • PDA2 antibody
  • PDI antibody
  • PDIA2 antibody
  • PDIA2_HUMAN antibody
  • PDIP antibody
  • PDIR antibody
  • Protein disulfide isomerase A2 antibody
  • Protein disulfide isomerase antibody
  • Protein disulfide isomerase associated 2 antibody
  • Protein disulfide isomerase family A member 2 antibody
  • Protein disulfide isomerase pancreatic antibody
  • Protein disulfide-isomerase A2 antibody
  • Rho GDP dissociation inhibitor gamma antibody
see all

Database links

see all

Anti-PDI antibody [RL90] - ER Marker images:

  Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)

Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)

Ab2792 positively staining dog MDCK cell ER (red) at 1/200. Staining was carried out in conjunction with goat anti mouse H and L ( Alexa 546) at 1/1000. The nuclei can be seen stained with Hoechst (blue)

This image is courtesy of an Abreview submitted by Kun Liu on 20 September 2005. We do not have any further information relating to this image.

  Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)

Immunocytochemistry - PDI antibody [RL90] - ER Marker (ab2792)

Ab2792 positively staining formaldehyde fixed human Hek293 cells (1/200). This Ab was used in conjunction with goat anti mouse Alexa Flour ® 546 (1/1500).

The nuclei has been atained with Hoechst.

This image is courtesy of an Abreview submitted by Kun Liu on 19th September 2005. We do not have any further information relating to this image.

See Abreview

  Western blot - PDI antibody [RL90] - ER Marker (ab2792)

Western blot - PDI antibody [RL90] - ER Marker (ab2792)



Predicted band size : 58 kDa

See Abreview

  Immunocytochemistry/ Immunofluorescence - PDI antibody [RL90] - ER Marker (ab2792)

Immunocytochemistry/ Immunofluorescence - PDI antibody [RL90] - ER Marker (ab2792)

ab2792 staining PDI from human HaCaT keratinocyte cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.25 % Triton ×100 and blocking with 2.5% BSA plus 1% goat serum for 1 hour at 40C was performed. Samples were incubated with primary antibody, diluted 1/100, for 1 hour at 240C. An Alexa Fluor ® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/1000 as secondary antibody.

This image is a courtesy of Anonymous Abreview

See Abreview

  Western blot - PDI antibody [RL90] - ER Marker (ab2792)

Western blot - PDI antibody [RL90] - ER Marker (ab2792)

Anti-PDI antibody [RL90] - ER Marker (ab2792) at 1/2000 dilution

Secondary
Donkey anti mouse IgG2a at 1/10000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 58 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)


Exposure time : 20 seconds

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-PDI antibody [RL90] - ER Marker (ab2792)

This product has been referenced in:

  • Wilhelmus MMet al. Presence of tissue transglutaminase in granular endoplasmic reticulum is characteristic of melanized neurons in Parkinson's disease brain. Brain Pathol 21:130-9 (2011). IHC-P; Human.Read more (PubMed: 20731657) »
  • Defays Aet al. BAD-LAMP is a novel biomarker of nonactivated human plasmacytoid dendritic cells. Blood 118:609-17 (2011).Read more (PubMed: 21642595) »

See all 35 publications for this product

Publishing research using ab2792? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"