PDI antibody [RL90] - ER Marker (ab2792)

Product Name 

PDI antibody [RL90] - ER Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [RL90] to PDI - ER Marker

Immunogen 

Full length native protein (purified) (Rat).

Reacts with 
(species key)

Hu, Ms, Rat, Dog, Dm, Hm, Mk

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Immunohistochemistry: 1/100 Perform heat mediated antigen retrieval via the microwave method in 10 mM Citrate, pH 6.0 for 10 minutes before commencing with IHC staining protocol. Allow tissues to cool to RT for 20 minutes and wash with tap water. A signal enhancing step may be necessary.
ICC/IF: Suggested working dilution of 1/100 (see PMID: 17308099).
WB: 1/1000
Immunoprecipitation: Use at an assay dependent concentration.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract.
Immunohistochemical staining of PDI in rat intestine yields a pattern consistent with cytoplasmic staining.
In immunoprecipitation procedures, this antibody has been shown to inhibit the activity of PDI in vitro.
This antibody has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

Positive control 
(see definition)

rat liver

Cellular localization 

Endoplasmic reticulum lumen.

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Tags & Cell Markers >> Subcellular Markers >> Organelles >> ER

Relevance 

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

RL90

Isotype 

IgG2a

Purity 

Ascites

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for PDI antibody [RL90] - ER Marker images, references, products related to ab2792 and other tools.

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References for PDI antibody [RL90] - ER Marker (ab2792)

  This product has been used in: (two most recent references)

Caporale M et al. The signal peptide of a simple retrovirus envelope functions as a posttranscriptional regulator of viral gene expression. J Virol 83:4591-604 (2009). ICC/IF; African Green Monkey. PubMed: 19244321

Li Y et al. HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport. J Cell Biol 185:1065-81 (2009). ICC/IF; Mouse. PubMed: 19528298

See all 31 publication references for this product.


If you publish research using ab2792 please let us know so that we can cite the reference on this datasheet.

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