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Anti-PDIA6 antibody (ab37756)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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Overview

Product name

Anti-PDIA6 antibody
See all PDIA6 products (4) ...

Description

Chicken polyclonal to PDIA6

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Dog, Human

Immunogen

Synthetic peptide, corresponding to amino acids 301-440 of Human PDIA6.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgY

Research areas

Signal Transduction >> Protein Trafficking >> ER Proteins

  • Western blot - PDIA6 antibody (ab37756)Western blot - PDIA6 antibody (ab37756) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab37756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/2000. Detects a band of approximately 70 kDa for the fusion protein (predicted molecular weight for the native protein: 48 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Protein disulfide isomerase (PDIA6) ia an enzyme belonging to the protein disulfide isomerase family. Structurally, it contains 2 thioredoxin domains and its function is to catalyze the rearrangement of -S-S- bonds in proteins.

Cellular localization

Endoplasmic reticulum

Alternative names

  • Endoplasmic reticulum protein 5 antibody
  • ERP5 antibody
  • P5 antibody
  • Protein disulfide isomerase A6 antibody
  • Protein disulfide isomerase associated 6 antibody
  • Protein disulfide isomerase family A member 6 antibody
  • Protein disulfide isomerase P5 antibody
  • Protein disulfide isomerase related protein antibody
  • Thioredoxin domain containing 7 (protein disulfide isomerase) antibody
  • Thioredoxin domain containing protein 7 antibody
  • TXNDC7 antibody
see all

Database links

see all

Anti-PDIA6 antibody images:

  Western blot - PDIA6 antibody (ab37756)

Western blot - PDIA6 antibody (ab37756)

Anti-PDIA6 antibody (ab37756) at 1/2000 dilution + E coli derived fusion protein at 2.5 µg

Secondary
Goat anti IgY HRP at 1/1000 dilution

Predicted band size : 48 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


The arrow next to the image points to the PDIA6 fusion protein.
Observed band size may differ from the predicted band size because the test antigen is a fusion protein.
A colorimetric method was used for signal development.

References for Anti-PDIA6 antibody (ab37756)

This product has been referenced in:

  • Estrada-Bernal A  et al. Functional complexity of the axonal growth cone: a proteomic analysis. PLoS One 7:e31858 (2012). WB, ICC/IF; Rat.Read more (PubMed: 22384089) »
  • Sørensen BS  et al. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother Oncol 92:443-9 (2009). WB; Human.Read more (PubMed: 19541378) »

See all 2 publications for this product

Publishing research using ab37756? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"