Overview

  • Product nameAnti-PDK2 antibody [EP1947Y]
    See all PDK2 primary antibodies
  • Description
    Rabbit monoclonal [EP1947Y] to PDK2
  • Tested applicationsWB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human PDK2.

  • Positive control
    • SH-SY-5Y cell lysate; human heart muscle tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Applications

Our Abpromise guarantee covers the use of ab76152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/50.
ICC 1/50.
Flow Cyt 1/100.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
    • Tissue specificityExpressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
    • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
      Contains 1 histidine kinase domain.
    • Cellular localizationMitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody
      • mitochondrial antibody
      • PDHK2 antibody
      • PDK2 antibody
      • PDK2_HUMAN antibody
      • Pyruvate dehydrogenase kinase isoform 2 antibody
      • Pyruvate dehydrogenase kinase, isozyme 2 antibody
      • Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
      see all

    Anti-PDK2 antibody [EP1947Y] images

    • ab76152 at 1/50 dilution staining PDK2 in human heart muscle by Immunohistochemistry, Paraffin-embedded tissue.
    • Anti-PDK2 antibody [EP1947Y] (ab76152) at 1/1000 dilution + SH-SY-5Y cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution
      developed using the ECL technique

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Overlay histogram showing HepG2 cells stained with ab76152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76152, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-PDK2 antibody [EP1947Y] (ab76152)

    ab76152 has not yet been referenced specifically in any publications.

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