Anti-PDK2 antibody [EP1947Y] (ab76152)

Overview

• Product nameAnti-PDK2 antibody [EP1947Y]See all PDK2 primary antibodies ...
• Description
  Rabbit monoclonal [EP1947Y] to PDK2
• Tested applicationsWB, IHC-P, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues near the N terminus of human PDK2.

• Positive controlSH-SY-5Y cell lysate; human heart muscle tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1947Y
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

Applications

Target

• FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
• Tissue specificityExpressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
• Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
  Contains 1 histidine kinase domain.
• Cellular localizationMitochondrion matrix.

Target information above from: UniProt accession Q15119 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5164 Human
  • Omim: 602525 Human
  • SwissProt: Q15119 Human
  • Unigene: 256667 Human

• Alternative names
  [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibodymitochondrial antibodyPDHK2 antibody

  PDK2 antibodyPDK2_HUMAN antibodyPyruvate dehydrogenase kinase isoform 2 antibodyPyruvate dehydrogenase kinase, isozyme 2 antibodyPyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody

  see all

Anti-PDK2 antibody [EP1947Y] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK2 antibody [EP1947Y] (ab76152)
  ab76152 at 1/50 dilution staining PDK2 in human heart muscle by Immunohistochemistry, Paraffin-embedded tissue.
• 

  Western blot - PDK2 antibody [EP1947Y] (ab76152)
  Anti-PDK2 antibody [EP1947Y] (ab76152) at 1/1000 dilution + SH-SY-5Y cell lysate at 10 µg
  
  Secondary
  goat anti-rabbit HRP at 1/1000 dilution
  developed using the ECL technique
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
• 

  Flow Cytometry - Anti-PDK2 antibody [EP1947Y] (ab76152)
  Overlay histogram showing HepG2 cells stained with ab76152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76152, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.