Overview
- Product nameAnti-PDK3 antibodySee all PDK3 primary antibodies ...
- DescriptionRabbit polyclonal to PDK3
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog - Immunogen
Synthetic peptide: LNRPSVGLVQ SWYMQSFLEL LEYENKSPED PQVLDNFLQV LIKVRNRHND , corresponding to N terminal amino acids 72-121 of Human PDK3
- Positive controlTransfected 293T cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab80646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| ELISA |
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
- Tissue specificityFound almost exclusively in heart and skeletal muscle.
- Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. - Cellular localizationMitochondrion matrix.
-
Database links
- Entrez Gene: 418595 Chicken
- Entrez Gene: 510841 Cow
- Entrez Gene: 5165 Human
- Entrez Gene: 236900 Mouse
- Entrez Gene: 296849 Rat
- Omim: 602526 Human
- SwissProt: Q15120 Human
- SwissProt: Q922H2 Mouse
- Unigene: 296031 Human
- Unigene: 12775 Mouse
see all
Target information above from: UniProt accession
Q15120
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial antibody
- mitochondrial antibodyPDK3 antibodyPDK3_HUMAN antibodyPyruvate dehydrogenase kinase isoenzyme 3 antibodyPyruvate dehydrogenase kinase isoform 3 antibodyPyruvate dehydrogenase kinase isozyme 3 antibodyPyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial antibody
see all
Anti-PDK3 antibody images
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Western blot - PDK3 antibody (ab80646)Anti-PDK3 antibody (ab80646) at 1 µg/ml + transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 47 kDa
Observed band size : 47 kDa
References for Anti-PDK3 antibody (ab80646)
ab80646 has not yet been referenced specifically in any publications.
