Overview

  • Product nameAnti-PDK3 antibodySee all PDK3 primary antibodies ...
  • Description
    Rabbit polyclonal to PDK3
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: LNRPSVGLVQ SWYMQSFLEL LEYENKSPED PQVLDNFLQV LIKVRNRHND , corresponding to N terminal amino acids 72-121 of Human PDK3

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityFound almost exclusively in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.
  • Target information above from: UniProt accession Q15120 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [lipoamide] kinase isozyme 3 mitochondrial antibody
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3 antibody
    • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial antibody
    • mitochondrial antibody
    • PDK3 antibody
    • PDK3_HUMAN antibody
    • Pyruvate dehydrogenase kinase isoenzyme 3 antibody
    • Pyruvate dehydrogenase kinase isoform 3 antibody
    • Pyruvate dehydrogenase kinase isozyme 3 antibody
    • Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial antibody
    see all

Anti-PDK3 antibody images

  • Anti-PDK3 antibody (ab80646) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

References for Anti-PDK3 antibody (ab80646)

ab80646 has not yet been referenced specifically in any publications.

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