![Western blot - PDK4 antibody [1C2BG5 ] (ab110336) image](#PDK4-Primary-antibodies-ab110336-1.jpg)
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- Secondary antibodies
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Product name
Anti-PDK4 antibody [1C2BG5 ]
See all PDK4 products (8) ...
Description
Mouse monoclonal [1C2BG5 ] to PDK4
Specificity
This antibody does not cross-react with other isoforms of Human PDK (i.e. PDK1, 2 or 3).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant human PDK4 protein
Positive control
HepG2 whole cell lysate and Human heart tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
1C2BG5
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - PDK4 antibody [1C2BG5 ] (ab110336)
![Western blot - PDK4 antibody [1C2BG5 ] (ab110336) image](/ps/datasheet/images/110/ab110336/PDK4-Primary-antibodies-ab110336-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab110336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa.
Target
Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Tissue specificity
Ubiquitous; highest levels of expression in heart and skeletal muscle.
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionQ16654
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4 antibody
- FLJ40832 antibody
see all
Database links
- Entrez Gene: 5166 Human
- Omim: 602527 Human
- SwissProt: Q16654 Human
- Unigene: 8364 Human
Anti-PDK4 antibody [1C2BG5 ] images:
Western blot - PDK4 antibody [1C2BG5 ] (ab110336)
All lanes : Anti-PDK4 antibody [1C2BG5 ] (ab110336) at 1 µg/ml
Lane 1 : HepG2 whole cell lysate at 10 µg
Lane 2 : Human heart tissue at 10 µg
Lane 3 : Recombinant Human PDK1 at 0.01 µg
Lane 4 : Recombinant Human PDK2 at 0.01 µg
Lane 5 : Recombinant Human PDK3 at 0.01 µg
Lane 6 : Recombinant Human PDK4 at 0.01 µg
Predicted band size : 46 kDa
References for Anti-PDK4 antibody [1C2BG5 ] (ab110336)
ab110336 has not yet been referenced specifically in any publications.
Publishing research using ab110336? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"