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Overview

  • Product namePDK4 protein (Active)See all PDK4 proteins and peptides ...
  • Protein descriptionRecombinant full length Human PDK4 (amino acids 11-411) with His tag at amino terminus.(UniProt ID = Q16654).
  • Uniprot accessionQ16654
  • Protein length401 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 90 % Purified via His tag
  • Purification notesPDK4 was purified by metal chelation column chromatography (Ni-IDA) using His Binding Columns.
  • Biological activityPDK4 activity was measured in a serial dilution of the recombinant protein and the minimum amount of the protein required to phosphorylate and fully inhibit PDH immunocaptured from given amount of human, bovine, rat or mouse samples was determined.
  • E.C. Number2.7.11.2
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferpH: 7.50
    Constituents: 0.14% Sodium phosphate, 1.46% Sodium chloride, 30% Glycerol, 0.08% DTT, 0.02% EDTA, 0.05% Triton-X-100
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab110356 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent concentration. See MitoSciences activity protocol.
    Sandwich ELISA sELISA: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab110671.

    • Protein info

    • Alternative names
        [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4FLJ40832mitochondrial
        Pdk4PDK4_HUMANPyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrialPyruvate dehydrogenase kinase 4Pyruvate dehydrogenase kinase isoenzyme 4Pyruvate dehydrogenase kinase isoform 4Pyruvate dehydrogenase kinase isozyme 4Pyruvate dehydrogenase kinase isozyme 4 mitochondrial
      see all
  • FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
  • Tissue specificityUbiquitous; highest levels of expression in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • Cellular localizationMitochondrion matrix.

    • Target information above from: UniProt accession Q16654 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for PDK4 protein (Active) (ab110356)

    ab110356 has not yet been referenced specifically in any publications.

    Publishing research using ab110356 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"