Anti-PDLIM2 antibody (ab68220)
Key features and details
- Mouse polyclonal to PDLIM2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDLIM2 antibody
See all PDLIM2 primary antibodies -
Description
Mouse polyclonal to PDLIM2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human PDLIM2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- PDLIM2 transfected 293T cell lysate and human liver tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 36, 38 kDa (predicted molecular weight: 37 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 36, 38 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
Cellular localization
Cytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin. - Information by UniProt
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Database links
- Entrez Gene: 64236 Human
- Omim: 609722 Human
- SwissProt: Q96JY6 Human
- Unigene: 632034 Human
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Alternative names
- FLJ34715 antibody
- MYSTIQUE antibody
- OTTHUMP00000162522 antibody
see all
Images
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Anti-PDLIM2 antibody (ab68220) at 1/500 dilution + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PDLIM2 antibody (ab68220) at 1/500 dilution
Lane 1 : PDLIM2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (4)
ab68220 has been referenced in 4 publications.
- Li Z et al. Central Nervous System-Infecting Pathogens Escherichia coli and Cryptococcus neoformans Exploit the Host Pdlim2 for Intracellular Traversal and Exocytosis in the Blood-Brain Barrier. Infect Immun 89:e0012821 (2021). PubMed: 34228504
- Jin M et al. Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin promotes inflammation in mouse testes: The critical role of Klotho in Sertoli cells. Toxicol Lett 295:134-143 (2018). WB . PubMed: 29885354
- Wurster KD et al. Inactivation of the putative ubiquitin-E3 ligase PDLIM2 in classical Hodgkin and anaplastic large cell lymphoma. Leukemia 31:602-613 (2017). WB ; Human . PubMed: 27538486
- Vanoirbeek E et al. PDLIM2 expression is driven by vitamin D and is involved in the pro-adhesion, and anti-migration and -invasion activity of vitamin D. Oncogene N/A:N/A (2013). Human . PubMed: 23584482