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Overview

  • Product namePDLIM2 protein (Tagged-His Tag)See all PDLIM2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 188-352 of Human PDLIM2 with N terminal His tag; 165 amino acids, 22kDa (19kDa predicted from sequence). UniProt ID = Q96JY6.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 126 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesDSAVLVLPPSPGPRSSRPSMDSEGGSLLLDEDSEVFKMLQ ENREGRAAPRQSSSFRLLQ EALEAEERGGTPAFLPSSL SPQSSLPASRALATPPKLHTCEKCSTSIANQAVRIQEGRY RHPGCYTCADCGLNLKMRGHFWVGDELYCEKHARQRYS APATLSSRA
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92038 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        FLJ34715MYSTIQUEOTTHUMP00000162522
        OTTHUMP00000205197OTTHUMP00000205198OTTHUMP00000205199OTTHUMP00000205200OTTHUMP00000205202OTTHUMP00000205203PDLI2_HUMANPdlim2PDZ and LIM domain 2 (mystique)PDZ and LIM domain protein 2PDZ-LIM protein mystiqueSLIM
      see all
  • FunctionProbable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity.
  • Sequence similaritiesContains 1 LIM zinc-binding domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localizationCytoplasm > cytoskeleton; Nucleus; Cytoplasm. Nucleus. May be partially nuclear and Cytoplasm > cytoskeleton. Colocalizes with beta-1 integrin (ITGB1) and alpha-actinin but not with paxillin.

    • Target information above from: UniProt accession Q96JY6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PDLIM2 protein (Tagged-His Tag) images

    • SDS-PAGE - PDLIM2 protein (Tagged-His Tag) (ab92038)
      SDS-PAGE - PDLIM2 protein (Tagged-His Tag) (ab92038)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PDLIM2 protein (Tagged-His Tag) (ab92038)

    ab92038 has not yet been referenced specifically in any publications.

    Publishing research using ab92038 ? Please let us know so that we can cite the reference in this datasheet

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