Anti-PDLIM7 antibody (ab86065)
Key features and details
- Rabbit polyclonal to PDLIM7
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDLIM7 antibody
See all PDLIM7 primary antibodies -
Description
Rabbit polyclonal to PDLIM7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Bat -
Immunogen
Synthetic peptide corresponding to a region between amino acids 125 and 175 of Human PDLIM7 (NP_005442.2)
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Positive control
- Hela cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway. -
Tissue specificity
Isoform 1 and isoform 2 are expressed ubiquitously, however, isoform 2 predominates in skeletal muscle, isoform 1 is more abundant in lung, spleen, leukocytes and fetal liver. -
Sequence similarities
Contains 3 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain. -
Domain
The LIM zinc-binding 2 (LIM 2) domain interacts with TBX4.
The LIM zinc-binding 3 (LIM 3) domain provides the structural basis for recognition of tyrosine-containing tight turn structures. This domain is necessary and sufficient for interaction with TBX5.
Anchored to cell periphery via its N-terminal PDZ domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with RET to the cell periphery and in some cytoskeletal components. Colocalizes with TPM2 near the Z line in muscle. Co-localizes with TBX4 and TBX5 to actin filaments. - Information by UniProt
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Database links
- Entrez Gene: 462308 Chimpanzee
- Entrez Gene: 533851 Cow
- Entrez Gene: 611246 Dog
- Entrez Gene: 101127125 Gorilla
- Entrez Gene: 100058356 Horse
- Entrez Gene: 9260 Human
- Entrez Gene: 706581 Rhesus monkey
- Omim: 605903 Human
see all -
Alternative names
- 1110003B01Rik antibody
- Enigma antibody
- LIM domain protein antibody
see all
Images
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All lanes : Anti-PDLIM7 antibody (ab86065) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Exposure time: 30 seconds -
Detection of PDLIM7 by Western blot of Immunprecipitate.
ab86065, at 1 µg/ml, staining PDLIM7 in HeLa whole cell lysate immunoprecipitated using ab86065 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab86065 has been referenced in 1 publication.
- Ajeian JN et al. Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl 10:51-7 (2016). PubMed: 26147903