Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)
All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml
Lane 1 : Molecular weight marker Lane 2 :Recombinant human PDP2 protein (ab110358) at 0.01 µg Lane 3 :Recombinant Human PDP1 protein (ab110357) at 0.01 µg Lane 4 :Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab110359) at 0.01 µg Lane 5 : HHH (Human heart homogenate) at 20 µg Lane 6 : HLH (Human liver homogenate) at 20 µg Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary All lanes : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa Observed band size: 60 kDa
Exposure time: 5 minutes
Predicted mature band size: 52 kDa Observed band size: 52 kDa
Note: The antibody recognizes both the mature (52 kDa) and the immature (60 kDa) versions of PDP2 protein. The immunogen was mature PDP2 protein, lacking the first 66 amino acids which are used as a transit peptide for mitochondrial targeting. The antibody produces a band against the endogenous protein at the mature weight in tissue homogenates, but not in cancer cells where the protein may fail to mature.
Ryan ZC et al. 1a,25-dihydroxyvitamin D3mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells. Biochem Biophys Res Commun496:746-752 (2018).
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