MitoSciences (MS1100)

Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)

Overview

  • Product nameAnti-PDP2 antibody [MS41A 3C3BC10]See all PDP2 primary antibodies ...
  • Description
    Mouse monoclonal [MS41A 3C3BC10] to PDP2
  • Specificityab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK.
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Full length protein corresponding to amino acids 1-529 of Human PDP2

  • Positive control
    • Recombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab133982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Note: This antibody works optimally in 5% Milk for all blocking and antibody steps.

Target

  • FunctionCatalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Sequence similaritiesBelongs to the PP2C family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 antibody
    • KIAA1348 antibody
    • mitochondrial antibody
    • PDP 2 antibody
    • Pdp2 antibody
    • PDP2_HUMAN antibody
    • PDPC 2 antibody
    • Pyruvate dehydrogenase phosphatase 2 antibody
    • Pyruvate dehydrogenase phosphatase catalytic subunit 2 antibody
    • pyruvate dehydrogenase phosphatase isoenzyme 2 antibody
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 2 antibody
    see all

Anti-PDP2 antibody [MS41A 3C3BC10] images

  • All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Human PDP2 full length protein (ab110358) at 0.01 µg
    Lane 3 : Human PDP1 full length protein (ab110357) at 0.01 µg
    Lane 4 : Active human Mitochondrial Pyruvate dehydrogenase kinase 1 full length protein (ab110359) at 0.01 µg
    Lane 5 : HHH (Human heart homogenate) at 20 µg
    Lane 6 : HLH (Human liver homogenate) at 20 µg
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
    Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 5 minutes

References for Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)

ab133982 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"