Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)

Overview

• Product nameAnti-PDP2 antibody [MS41A 3C3BC10]See all PDP2 primary antibodies ...
• Description
  Mouse monoclonal [MS41A 3C3BC10] to PDP2
• Specificityab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Full length protein corresponding to amino acids 1-529 of Human PDP2

  Run BLAST with Run BLAST with
• Positive controlRecombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: 99% HBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesNear homogeneity as judged by SDS-PAGE. ab133982 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
• Clonality Monoclonal
• Clone numberMS41A 3C3BC10
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

Applications

Target

• FunctionCatalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
• Sequence similaritiesBelongs to the PP2C family.
• Cellular localizationMitochondrion matrix.

Target information above from: UniProt accession Q9P2J9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 57546 Human
  • SwissProt: Q9P2J9 Human
  • Unigene: 632214 Human
  • Unigene: 654693 Human

• Alternative names
  [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 antibodyKIAA1348 antibodymitochondrial antibody

  PDP 2 antibodyPdp2 antibodyPDP2_HUMAN antibodyPDPC 2 antibodyPyruvate dehydrogenase phosphatase 2 antibodyPyruvate dehydrogenase phosphatase catalytic subunit 2 antibodypyruvate dehydrogenase phosphatase isoenzyme 2 antibodyPyruvate dehydrogenase phosphatase, catalytic subunit 2 antibody

  see all

Anti-PDP2 antibody [MS41A 3C3BC10] images

• 

  Western blot - Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)
  All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml
  
  Lane 1 : Molecular weight marker
  Lane 2 : PDP2 protein (ab110358) at 0.01 µg
  Lane 3 : PDP1 protein (ab110357) at 0.01 µg
  Lane 4 : Mitochondrial Pyruvate dehydrogenase kinase 1 protein (Active) (ab110359) at 0.01 µg
  Lane 5 : HHH (Human heart homogenate) at 20 µg
  Lane 6 : HLH (Human liver homogenate) at 20 µg
  Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
  Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
  Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
  Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 60 kDa
  Observed band size : 60 kDa
  
  
  Exposure time : 5 minutes