Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982)

Overview

  • Product name
    Anti-PDP2 antibody [MS41A 3C3BC10]
    See all PDP2 primary antibodies
  • Description
    Mouse monoclonal [MS41A 3C3BC10] to PDP2
  • Specificity
    ab133982 does not react with Human Recombinant PDP1 or Human Recombinant PDK.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Full length protein corresponding to amino acids 1-529 of Human PDP2

  • Positive control
    • Recombinant PDP2 protein, Human heart or liver homogenate, HeLa, HEK293, HepG2 and jurkat whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab133982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Note: This antibody works optimally in 5% Milk for all blocking and antibody steps.

Target

  • Function
    Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
  • Sequence similarities
    Belongs to the PP2C family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2 antibody
    • KIAA1348 antibody
    • mitochondrial antibody
    • PDP 2 antibody
    • Pdp2 antibody
    • PDP2_HUMAN antibody
    • PDPC 2 antibody
    • PPM2B antibody
    • PPM2C2 antibody
    • Protein phosphatase 2C magnesium dependent catalytic subunit 2 antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 2B antibody
    • Pyruvate dehydrogenase phosphatase 2 antibody
    • Pyruvate dehydrogenase phosphatase catalytic subunit 2 antibody
    • pyruvate dehydrogenase phosphatase isoenzyme 2 antibody
    • Pyruvate dehydrogenase phosphatase, catalytic subunit 2 antibody
    see all

Images

  • All lanes : Anti-PDP2 antibody [MS41A 3C3BC10] (ab133982) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Recombinant human PDP2 protein (ab110358) at 0.01 µg
    Lane 3 : Recombinant Human PDP1 protein (ab110357) at 0.01 µg
    Lane 4 : Recombinant human Mitochondrial Pyruvate dehydrogenase kinase 1 protein (ab110359) at 0.01 µg
    Lane 5 : HHH (Human heart homogenate) at 20 µg
    Lane 6 : HLH (Human liver homogenate) at 20 µg
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 8 : HEK293 (Human embryonic kidney 293 cell line) Whole Cell Lysate at 10 µg
    Lane 9 : HepG2 (Human hepatocellular carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 10 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 5 minutes

References

ab133982 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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