Overview

  • Product nameAnti-PDPK1 antibody [EP569Y]See all PDPK1 primary antibodies ...
  • Description
    Rabbit monoclonal [EP569Y] to PDPK1
  • SpecificityThis antibody detects PDK1 both when phosphorylated and non-phosphorylated on Tyrosine 373 and 376.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Tyr373 and Tyr376 of human PDPK1.

  • Positive control
    • 293 cell lysate or human prostate carcinoma tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab52893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IHC-P Use at an assay dependent dilution.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    • Tissue specificityAppears to be expressed ubiquitously.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK 1 antibody
      • PDK1 antibody
      • PDPK 1 antibody
      • PDPK1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PkB like antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Anti-PDPK1 antibody [EP569Y] images

    • Ab52893 (1:100) staining human PDPK1 in human prostate carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Anti-PDPK1 antibody [EP569Y] (ab52893) at 1/1000 dilution + 293 cell lysate

      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    References for Anti-PDPK1 antibody [EP569Y] (ab52893)

    This product has been referenced in:
    • Shen X  et al. PDK1 Recruitment to the SHPS-1 Signaling Complex Enhances Insulin-like Growth Factor-I-stimulated AKT Activation and Vascular Smooth Muscle Cell Survival. J Biol Chem 285:29416-24 (2010). WB ; Pig . Read more (PubMed: 20643654) »
    • Block K  et al. Nox4 NAD(P)H oxidase mediates Src-dependent tyrosine phosphorylation of PDK-1 in response to angiotensin II: role in mesangial cell hypertrophy and fibronectin expression. J Biol Chem 283:24061-76 (2008). WB ; Rat . Read more (PubMed: 18559349) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"