The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
Use at an assay dependent concentration.
Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Tissue specificityAppears to be expressed ubiquitously.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. Contains 1 PH domain. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
Predicted band size : 63 kDa Observed band size : 63 kDa
References for Anti-PDPK1 antibody [EP569Y] (ab52893)
This product has been referenced in:
Lelliott CJ et al. Monoclonal antibody targeting of fibroblast growth factor receptor 1c ameliorates obesity and glucose intolerance via central mechanisms. PLoS One9:e112109 (2014).
Read more (PubMed: 25427253) »
Shen X et al. PDK1 Recruitment to the SHPS-1 Signaling Complex Enhances Insulin-like Growth Factor-I-stimulated AKT Activation and Vascular Smooth Muscle Cell Survival. J Biol Chem285:29416-24 (2010).
Read more (PubMed: 20643654) »