Overview
- Product nameAnti-PDPK1 antibody [EPR245]See all PDPK1 primary antibodies ...
- DescriptionRabbit monoclonal [EPR245] to PDPK1
- Tested applicationsWB, IHC-P, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthetic peptide corresponding to residues in the N-terminus of Human PDPK1.
- Positive control293T and PC12 cell lysates; Human breast carcinoma tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR245
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab109253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/10000. Predicted molecular weight: 63 kDa. |
| IHC-P | IHC-P: 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| ICC | ICC: 1/100 - 1/250. |
Target
- FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
- Tissue specificityAppears to be expressed ubiquitously.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. - Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
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Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Entrez Gene: 81745 Rat
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- SwissProt: O55173 Rat
- Unigene: 459691 Human
- Unigene: 10504 Mouse
- Unigene: 10905 Rat
see all
Target information above from: UniProt accession
O15530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 3 phosphoinositide dependent protein kinase 1 antibody3-phosphoinositide-dependent protein kinase 1 antibodyhPDK 1 antibody
- hPDK1 antibodyMGC20087 antibodyMGC35290 antibodyOTTHUMP00000159109 antibodyOTTHUMP00000159110 antibodyOTTHUMP00000174525 antibodyPDK 1 antibodyPDK1 antibodyPdpk1 antibodyPDPK1_HUMAN antibodyPkB kinase antibodyPkB kinase like gene 1 antibodyPkB like 1 antibodyPRO0461 antibodyProtein kinase antibody
see all
Anti-PDPK1 antibody [EPR245] images
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![Western blot - PDPK1 antibody [EPR245] (ab109253)](http://a.abcam.com/ps/datasheet/images/109/ab109253/PDPK1-Primary-antibodies-ab109253-1.jpg)
Western blot - PDPK1 antibody [EPR245] (ab109253)All lanes : Anti-PDPK1 antibody [EPR245] (ab109253) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 63 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 antibody [EPR245] (ab109253)](http://a.abcam.com/ps/datasheet/images/109/ab109253/PDPK1-Primary-antibodies-ab109253-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 antibody [EPR245] (ab109253)ab109253, at a 1/500 dilution, staining PDPK1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
References for Anti-PDPK1 antibody [EPR245] (ab109253)
ab109253 has not yet been referenced specifically in any publications.
