RabMAb

Anti-PDPK1 antibody [EPR245] (ab109253)

Overview

  • Product nameAnti-PDPK1 antibody [EPR245]
    See all PDPK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR245] to PDPK1
  • Tested applicationsWB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the N-terminus of Human PDPK1.

  • Positive control
    • 293T and PC12 cell lysates; Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    • Tissue specificityAppears to be expressed ubiquitously.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 phosphoinositide dependent protein kinase 1 antibody
      • 3-phosphoinositide-dependent protein kinase 1 antibody
      • hPDK 1 antibody
      • hPDK1 antibody
      • MGC20087 antibody
      • MGC35290 antibody
      • OTTHUMP00000159109 antibody
      • OTTHUMP00000159110 antibody
      • OTTHUMP00000174525 antibody
      • PDK 1 antibody
      • PDK1 antibody
      • Pdpk1 antibody
      • PDPK1_HUMAN antibody
      • PDPK2 antibody
      • PkB kinase antibody
      • PkB kinase like gene 1 antibody
      • PkB like 1 antibody
      • PRO0461 antibody
      • Protein kinase antibody
      see all

    Anti-PDPK1 antibody [EPR245] images

    • All lanes : Anti-PDPK1 antibody [EPR245] (ab109253) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 63 kDa
    • ab109253, at a 1/500 dilution, staining PDPK1 in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-PDPK1 antibody [EPR245] (ab109253)

    ab109253 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (head and neck cancer)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 20 µg
    Treatment DCA 20mM for 24hs
    Specification head and neck cancer
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 02 2015

    Application Western blot
    Sample Mouse Cell lysate - whole cell (head and neck cancer)
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 10%)
    Loading amount 30 µg
    Treatment DCA 20mM for 24hs
    Specification head and neck cancer
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Miss. jin young park

    Verified customer

    Submitted May 22 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (head and neck cancer)
    Permeabilization Yes - triton x 100
    Specification head and neck cancer
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 28 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"