Overview

  • Product nameAnti-PDPK1 antibody - N-terminalSee all PDPK1 primary antibodies ...
  • Description
    Rabbit polyclonal to PDPK1 - N-terminal
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human PDPK1 conjugated to KLH.

  • Positive control
    • HL-60 cell lysate. Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificityAppears to be expressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Target information above from: UniProt accession O15530 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK 1 antibody
    • PDK1 antibody
    • PDPK 1 antibody
    • PDPK1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PkB like antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Anti-PDPK1 antibody - N-terminal images

  • Anti-PDPK1 antibody - N-terminal (ab71757) at 1/100 dilution + HL-60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • ab71757 at 1/50 dilution, staining PDPK1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-PDPK1 antibody - N-terminal (ab71757)

ab71757 has not yet been referenced specifically in any publications.

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