Anti-PDPK1 antibody - N-terminal (ab71757)

Overview

• Product nameAnti-PDPK1 antibody - N-terminalSee all PDPK1 primary antibodies ...
• Description
  Rabbit polyclonal to PDPK1 - N-terminal
• Tested applicationsELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide from the N-terminal region of human PDPK1 conjugated to KLH.

• Positive controlHL-60 cell lysate. Human hepatocarcinoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesab71757 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Assembly

Applications

Target

• FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
• Tissue specificityAppears to be expressed ubiquitously.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
• Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Target information above from: UniProt accession O15530 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5170 Human
  • Entrez Gene: 5170 Human
  • Entrez Gene: 5170 Human
  • Omim: 605213 Human
  • SwissProt: O15530 Human
  • SwissProt: O15530 Human
  • SwissProt: O15530 Human
  • Unigene: 459691 Human

• Alternative names
  3 phosphoinositide dependent protein kinase 1 antibody3-phosphoinositide-dependent protein kinase 1 antibodyhPDK 1 antibody

  hPDK1 antibodyMGC20087 antibodyMGC35290 antibodyOTTHUMP00000159109 antibodyOTTHUMP00000159110 antibodyOTTHUMP00000174525 antibodyPDK 1 antibodyPDK1 antibodyPDPK 1 antibodyPDPK1 antibodyPDPK1_HUMAN antibodyPkB kinase antibodyPkB kinase like gene 1 antibodyPkB like 1 antibodyPkB like antibodyPRO0461 antibodyProtein kinase antibody

  see all

Anti-PDPK1 antibody - N-terminal images

• 

  Western blot - PDPK1 antibody - N-terminal (ab71757)
  Anti-PDPK1 antibody - N-terminal (ab71757) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
  
  Secondary
  HRP-anti-rabbit
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 antibody - N-terminal (ab71757)
  ab71757 at 1/50 dilution, staining PDPK1 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.