Overview

  • Product name
    Anti-PDPK1 antibody
    See all PDPK1 primary antibodies
  • Description
    Rabbit polyclonal to PDPK1
  • Host species
    Rabbit
  • Specificity
    ab19034 recognises PDPK1 (Phosphoinositide-dependent protein kinase 1).
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to C terminal of Human PDPK1

Properties

Applications

Our Abpromise guarantee covers the use of ab19034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 63 kDa.
IP Use a concentration of 10 - 20 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificity
    Appears to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localization
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

References

ab19034 has not yet been referenced specifically in any publications.

Publishing research using ab19034? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab19034.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up