Anti-PDPK1 antibody (ab19034)

Overview

• Product name
  Anti-PDPK1 antibody
  See all PDPK1 primary antibodies
• Description
  Rabbit polyclonal to PDPK1
• Specificity
  ab19034 recognises PDPK1 (Phosphoinositide-dependent protein kinase 1).
• Tested applications
  Suitable for: WB, IP, IHC-Pmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide, corresponding to C terminal of Human PDPK1

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, 1% BSA, PBS
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Assembly

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
• Tissue specificity
  Appears to be expressed ubiquitously.
• Sequence similarities
  Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• Post-translational
  modifications
  Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
• Cellular localization
  Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

• Target information above from: UniProt accession O15530 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 5170 Human
  • Entrez Gene: 18607 Mouse
  • Entrez Gene: 81745 Rat
  • Omim: 605213 Human
  • SwissProt: O15530 Human
  • SwissProt: Q9Z2A0 Mouse
  • SwissProt: O55173 Rat
  • Unigene: 459691 Human

  • Unigene: 10504 Mouse
  • Unigene: 10905 Rat

  see all
• Alternative names
  • 3 phosphoinositide dependent protein kinase 1 antibody
  • 3-phosphoinositide-dependent protein kinase 1 antibody
  • hPDK 1 antibody

  • hPDK1 antibody
  • MGC20087 antibody
  • MGC35290 antibody
  • OTTHUMP00000159109 antibody
  • OTTHUMP00000159110 antibody
  • OTTHUMP00000174525 antibody
  • PDK1 antibody
  • Pdpk1 antibody
  • PDPK1_HUMAN antibody
  • PDPK2 antibody
  • PDPK2P antibody
  • PkB kinase antibody
  • PkB kinase like gene 1 antibody
  • PkB like 1 antibody
  • PRO0461 antibody
  • Protein kinase antibody

  see all

Protocols

• Western blot protocols
• Immunoprecipitation protocols
• Immunohistochemistry protocols