Overview
- Product nameAnti-PDPK1 antibodySee all PDPK1 primary antibodies ...
- DescriptionRabbit polyclonal to PDPK1
- SpecificityThis antibody is primarily directed against 60,000 MW human PDPK1 and is useful in determining its presence in various assays. It predominantly recognizes a 60 kDa band on Western blots.
- Tested applicationsWB more details
- Species reactivityReacts with: Human
- Immunogen
Peptide sequence: RKIQEVWRQRYQS (amino acids 301-312).
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
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Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab2495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB |
(previously used at this dilution to detect PDK 1 in lysates derived from Sf9 insect cells infected with virus expressing myc-tagged human PDK 1.
Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation.
Target
- FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
- Tissue specificityAppears to be expressed ubiquitously.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. - Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
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Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- Unigene: 459691 Human
Target information above from: UniProt accession
O15530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 3 Phosphoinositide Dependent Protein Kinase 1 antibody3-phosphoinositide-dependent protein kinase 1 antibodyhPDK 1 antibody
- hPDK1 antibodyMGC20087 antibodyMGC35290 antibodyOTTHUMP00000159109 antibodyOTTHUMP00000159110 antibodyOTTHUMP00000174525 antibodyPDK1 antibodyPDPK1 antibodyPDPK1_HUMAN antibodyPkB kinase antibodyPkB kinase like gene 1 antibodyPkB like 1 antibodyPRO0461 antibodyProtein kinase antibody
see all
Anti-PDPK1 antibody images
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Western blot - PDPK1 antibody (ab2495)Immunoblot of Sf9 insect cell lysate. Affinity purified anti-Human PDPK1 detects 60 kDa human PDPK1 in lysates from myc-tagged human PDPK1 virus infected Sf9 cells. A 1/750 dilution is suggested followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L] and chemiluminescent detection. Other detection systems will yield similar results.
References for Anti-PDPK1 antibody (ab2495)
ab2495 has not yet been referenced specifically in any publications.
