Overview

  • Product nameAnti-PDPK1 antibodySee all PDPK1 primary antibodies ...
  • Description
    Rabbit polyclonal to PDPK1
  • SpecificityPDPK1 antibody detects endogenous levels of PDPK1 protein around the phosphorylation site of Serine 241.
  • Tested applicationsWB, ELISA, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.

  • Positive control
    • MDA-MB-435 cells treated with EGF and COS7 cells treated with TNF alpha. Detected in heart, testes and kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab31406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Predicted molecular weight: 63 kDa.
ELISA 1/10000.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificityAppears to be expressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Target information above from: UniProt accession O15530 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 3 Phosphoinositide Dependent Protein Kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • PDPK1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Anti-PDPK1 antibody images

  • ab31406 staining PDPK1 in breast carcinoma by Immunohistochemistry, Paraffin Embedded Tissue.

    Left image : ab31406 (1/200)

    Right image : ab31406 (1/200) pre-incubated with blocking peptide (negative control).

  • ab31406 at 1/200 staining mouse cystic kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked in serum for 25 minutes before incubation with the antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab31406 staining rat heart tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking in 1% serum for 25 minutes at 22°C.  The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 22°C.  A HRP-conjugated goat anti-rabbit antibody was used as the secodary.

    See Abreview

  • Anti-PDPK1 antibody (ab31406) at 1/1000 dilution + Active human PDPK1 full length protein (ab60834) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Anti-PDPK1 antibody (ab31406)

This product has been referenced in:
  • Kucherlapati MH  et al. Genotype directed therapy in murine mismatch repair deficient tumors. PLoS One 8:e68817 (2013). Read more (PubMed: 23935891) »
  • So T  et al. OX40 Complexes with Phosphoinositide 3-Kinase and Protein Kinase B (PKB) To Augment TCR-Dependent PKB Signaling. J Immunol 186:3547-55 (2011). Read more (PubMed: 21289304) »

See all 5 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for onevial of ab31406.

To check the status of the order please contact ou...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A2058 Melanoma cells)
Specification A2058 Melanoma cells
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Anita Boyapati

Verified customer

Submitted Jun 30 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2007

Application Western blot
Sample Human Cell lysate - whole cell (U2OS cell line)
Loading amount 30 µg
Specification U2OS cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart (muscle))
Specification Heart (muscle)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cystic Kidney)
Specification Cystic Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenal tumour (VHL mutant))
Specification Adrenal tumour (VHL mutant)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"