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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Read our guarantee »Anti-PDPK1 antibody
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Rabbit polyclonal to PDPK1
PDPK1 antibody detects endogenous levels of PDPK1 protein around the phosphorylation site of Serine 241.
WB, ELISA, IHC-P, ICC/IFmore details
Reacts with
Mouse, Rat, Human
Synthetic non-phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
MDA-MB-435 cells treated with EGF and COS7 cells treated with TNF alpha. Detected in heart, testes and kidney tissue.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody (ab31406)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)
(enlarge)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)
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Our Abpromise guarantee covers the use of ab31406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 63 kDa.
ELISA: 1/10000.
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: 1/100.
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Appears to be expressed ubiquitously.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
Target information above from: UniProt accessionO15530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody (ab31406)

ab31406 staining PDPK1 in breast carcinoma by Immunohistochemistry, Paraffin Embedded Tissue.
Left image : ab31406 (1/200)
Right image : ab31406 (1/200) pre-incubated with blocking peptide (negative control).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)

ab31406 at 1/200 staining mouse cystic kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked in serum for 25 minutes before incubation with the antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)

ab31406 staining rat heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking in 1% serum for 25 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 22°C. A HRP-conjugated goat anti-rabbit antibody was used as the secodary.
This image is courtesy of an anonymous Abreview
Western blot - Anti-PDPK1 antibody (ab31406)

Anti-PDPK1 antibody (ab31406) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
This product has been referenced in:
See all 4 publications for this product
Publishing research using ab31406? Please let us know so that we can cite the reference in this datasheet
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ab31406 staining PDPK1 in breast carcinoma by Immunohistochemistry, Paraffin Embedded Tissue.
Left image : ab31406 (1/200)
Right image : ab31406 (1/200) pre-incubated with blocking peptide (negative control).

ab31406 at 1/200 staining mouse cystic kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked in serum for 25 minutes before incubation with the antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview

ab31406 staining rat heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking in 1% serum for 25 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 22°C. A HRP-conjugated goat anti-rabbit antibody was used as the secodary.
This image is courtesy of an anonymous Abreview
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