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Synthetic non-phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
Our Abpromise guarantee covers the use of ab31406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 63 kDa.|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Left image : ab31406 (1/200)
Right image : ab31406 (1/200) pre-incubated with blocking peptide (negative control).
ab31406 staining rat heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking in 1% serum for 25 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 22°C. A HRP-conjugated goat anti-rabbit antibody was used as the secodary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"