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Product name
Anti-PDPK1 antibody
See all PDPK1 products (13) ...
Description
Rabbit polyclonal to PDPK1
Specificity
PDPK1 antibody detects endogenous levels of PDPK1 protein around the phosphorylation site of Serine 241.
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human PDPK1 around the phosphorylation site of Serine 241.
Positive control
MDA-MB-435 cells treated with EGF and COS7 cells treated with TNF alpha. Detected in heart, testes and kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody (ab31406)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 63 kDa.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
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IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: 1/100.
Target
Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
Tissue specificity
Appears to be expressed ubiquitously.
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
Target information above from: UniProt accessionO15530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3 Phosphoinositide Dependent Protein Kinase 1 antibody
- 3-phosphoinositide-dependent protein kinase 1 antibody
- hPDK 1 antibody
see all
Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Entrez Gene: 81745 Rat
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- SwissProt: O55173 Rat
- Unigene: 459691 Human
see all
Anti-PDPK1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDPK1 antibody (ab31406)
ab31406 staining PDPK1 in breast carcinoma by Immunohistochemistry, Paraffin Embedded Tissue.
Left image : ab31406 (1/200)
Right image : ab31406 (1/200) pre-incubated with blocking peptide (negative control).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)
ab31406 at 1/200 staining mouse cystic kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked in serum for 25 minutes before incubation with the antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDK1 antibody (ab31406)
ab31406 staining rat heart tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking in 1% serum for 25 minutes at 22°C. The primary antibody was diluted 1/200 and incubated with the sample for 30 minutes at 22°C. A HRP-conjugated goat anti-rabbit antibody was used as the secodary.
This image is courtesy of an anonymous Abreview
Western blot - Anti-PDPK1 antibody (ab31406)
Anti-PDPK1 antibody (ab31406) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Anti-PDPK1 antibody (ab31406)
This product has been referenced in:
- So T et al. OX40 Complexes with Phosphoinositide 3-Kinase and Protein Kinase B (PKB) To Augment TCR-Dependent PKB Signaling. J Immunol 186:3547-55 (2011).Read more (PubMed: 21289304) »
- Lu Z et al. 3-phosphoinositide-dependent protein kinase-1 regulates proliferation and survival of cancer cells with an activated mitogen-activated protein kinase pathway. Mol Cancer Res 8:421-32 (2010). WB; Human.Read more (PubMed: 20197379) »
See all 4 publications for this product
Publishing research using ab31406? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"