Overview

  • Product nameAnti-PDPK1 antibodySee all PDPK1 primary antibodies ...
  • Description
    Rabbit polyclonal to PDPK1
  • Specificityab45374 detects a 65 kDa protein corresponding to the apparent molecular mass of PDPK1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: RTTSQLYDAVPIQS, corresponding to N terminal amino acids 3-16 of Human PDPK1

  • Positive control
    • Human A431 cells and mouse brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab45374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa).
ELISA 1/2000.

Target

  • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificityAppears to be expressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Target information above from: UniProt accession O15530 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 3 Phosphoinositide Dependent Protein Kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • PDPK1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

References for Anti-PDPK1 antibody (ab45374)

ab45374 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for onevial of ab31406.

To check the status of the order please contact ou...

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Thank you for your enquiry. Antibodies ab45374 and ab45375 were made from the N-terminus of human PDK-1 (accession #O15530). This region is not found in the drosophila PDK sequences found in the NCBI database, but if they would like do your own BLAS...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"