Overview

  • Product nameAnti-PDPK1 antibody
    See all PDPK1 primary antibodies
  • Description
    Rabbit polyclonal to PDPK1
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues surrounding S410 of human PDPK1 conjugated to KLH

  • Positive control
    • 293 cell lysates transiently transfected with the PDPK1 gene. Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/10 - 1/50.

Target

  • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificityAppears to be expressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK 1 antibody
    • PDK1 antibody
    • PDPK 1 antibody
    • PDPK1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PkB like antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Anti-PDPK1 antibody images

  • All lanes : Anti-PDPK1 antibody (ab71421) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysates
    Lane 2 : 293 cell lysates transiently transfected with the PDPK1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ab71421 at 1/10 dilution, staining PDPK1 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-PDPK1 antibody (ab71421)

ab71421 has not yet been referenced specifically in any publications.

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