Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460)

Overview

• Product nameAnti-PDPK1 (phospho S241) antibody [EPR336(2)]See all PDPK1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR336(2)] to PDPK1 (phospho S241)
• Specificityab109460 only detects PDPK1 phosphorylated at Serine 241.
• Tested applicationsWB, IP, IHC-P, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A phospho specific peptide corresponding to residues surrounding serine 241 of Human PDPK1.

• Positive control293T cell lysate; Human breast carcinoma tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR336(2)
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Assembly

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
• Tissue specificityAppears to be expressed ubiquitously.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
  Contains 1 PH domain.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
• Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Target information above from: UniProt accession O15530 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5170 Human
  • Entrez Gene: 5170 Human
  • Entrez Gene: 5170 Human
  • Entrez Gene: 18607 Mouse
  • Entrez Gene: 81745 Rat
  • Omim: 605213 Human
  • SwissProt: O15530 Human
  • SwissProt: O15530 Human

  • SwissProt: O15530 Human
  • SwissProt: Q9Z2A0 Mouse
  • SwissProt: O55173 Rat
  • Unigene: 459691 Human
  • Unigene: 10504 Mouse
  • Unigene: 10905 Rat

  see all

• Alternative names
  3 phosphoinositide dependent protein kinase 1 antibody3-phosphoinositide-dependent protein kinase 1 antibodyhPDK 1 antibody

  hPDK1 antibodyMGC20087 antibodyMGC35290 antibodyOTTHUMP00000159109 antibodyOTTHUMP00000159110 antibodyOTTHUMP00000174525 antibodyPDK1 antibodyPdpk1 antibodyPDPK1_HUMAN antibodyPkB kinase antibodyPkB kinase like gene 1 antibodyPkB like 1 antibodyPRO0461 antibodyProtein kinase antibody

  see all

Anti-PDPK1 (phospho S241) antibody [EPR336(2)] images

• 

  Western blot - PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460)
  All lanes : Anti-PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460) at 1/1000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : 293T cell lysate treated with Lambda Phosphatase
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-labeled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 63 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDPK1 (phospho S241) antibody [EPR336(2)] (ab109460)
  ab109460, at a 1/100 dilution, staining PDPK1 (phospho S241) in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.