Overview

  • Product nameAnti-PDPK1 (phospho S241) antibody
    See all PDPK1 primary antibodies
  • Description
    Rabbit polyclonal to PDPK1 (phospho S241)
  • SpecificityThis antibody detects endogenous levels of PDPK1 only when phosphorylated at Serine 241.
  • Tested applicationsWB, ELISA, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide (Human) derived from PDPK1 around the phosphorylation site of Serine 241.

  • Positive control
    • WB: extracts from MDA MB 435 cells and COS7 cells. IHC-P: breast carcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide.
    Constituents: 50% Glycerol, PBS (without calcium and magnesium ions), 150mM Sodium chloride, pH 7.4.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab32800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificityAppears to be expressed ubiquitously.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 Phosphoinositide Dependent Protein Kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • PDPK1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

Anti-PDPK1 (phospho S241) antibody images

  • All lanes : Anti-PDPK1 (phospho S241) antibody (ab32800) at 1/1000 dilution

    Lane 1 : MDA MB 435 cell extract treated with EGF.
    Lane 2 : COS7 cell extract treated with TNF.


    Predicted band size : 63 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Ab32800, at a 1/50 dilution, staining PDPK1 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.
    Left image: untreated.
    Right image: treated with synthesized phosphopeptide.

References for Anti-PDPK1 (phospho S241) antibody (ab32800)

This product has been referenced in:
  • Baryawno N  et al. Small-molecule inhibitors of phosphatidylinositol 3-kinase/Akt signaling inhibit Wnt/beta-catenin pathway cross-talk and suppress medulloblastoma growth. Cancer Res 70:266-76 (2010). IHC-P ; Human . Read more (PubMed: 20028853) »
  • Rodriguez OC  et al. A reduction in Pten tumor suppressor activity promotes ErbB-2-induced mouse prostate adenocarcinoma formation through the activation of signaling cascades downstream of PDK1. Am J Pathol 174:2051-60 (2009). IHC-P, WB ; Human, Mouse . Read more (PubMed: 19443706) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"