Anti-PDPK1 (phospho S241) antibody (ab32800)
Overview
- Product nameAnti-PDPK1 (phospho S241) antibodySee all PDPK1 primary antibodies ...
- DescriptionRabbit polyclonal to PDPK1 (phospho S241)
- SpecificityThis antibody detects endogenous levels of PDPK1 only when phosphorylated at Serine 241.
- Tested applicationsWB, ELISA, IHC-P, IP more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic phosphopeptide (Human) derived from PDPK1 around the phosphorylation site of Serine 241.
- Positive controlWB: extracts from MDA MB 435 cells and COS7 cells. IHC-P: breast carcinoma.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide.
Constituents: 50% Glycerol, PBS (without calcium and magnesium ions), 150mM Sodium chloride, pH 7.4. -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab32800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa). |
| ELISA | ELISA: 1/20000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| IP | IP: Use at an assay dependent dilution. |
Target
- FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
- Tissue specificityAppears to be expressed ubiquitously.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. - Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
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Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Entrez Gene: 81745 Rat
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- SwissProt: O55173 Rat
- Unigene: 459691 Human
- Unigene: 10504 Mouse
- Unigene: 10905 Rat
see all
Target information above from: UniProt accession
O15530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 3 Phosphoinositide Dependent Protein Kinase 1 antibody3-phosphoinositide-dependent protein kinase 1 antibodyhPDK 1 antibody
- hPDK1 antibodyMGC20087 antibodyMGC35290 antibodyOTTHUMP00000159109 antibodyOTTHUMP00000159110 antibodyOTTHUMP00000174525 antibodyPDK1 antibodyPDPK1 antibodyPDPK1_HUMAN antibodyPkB kinase antibodyPkB kinase like gene 1 antibodyPkB like 1 antibodyPRO0461 antibodyProtein kinase antibody
see all
Anti-PDPK1 (phospho S241) antibody images
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Western blot - PDPK1 (phospho S241) antibody (ab32800)All lanes : Anti-PDPK1 (phospho S241) antibody (ab32800) at 1/1000 dilution
Lane 1 : MDA MB 435 cell extract treated with EGF.
Lane 2 : COS7 cell extract treated with TNF.
Predicted band size : 63 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - PDPK1 (phospho S241) antibody (ab32800)Ab32800, at a 1/50 dilution, staining PDPK1 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.
Left image: untreated.
Right image: treated with synthesized phosphopeptide.
References for Anti-PDPK1 (phospho S241) antibody (ab32800)
This product has been referenced in:
- Baryawno N et al. Small-molecule inhibitors of phosphatidylinositol 3-kinase/Akt signaling inhibit Wnt/beta-catenin pathway cross-talk and suppress medulloblastoma growth. Cancer Res 70:266-76 (2010). IHC-P ; Human . Read more (PubMed: 20028853) »
- Rodriguez OC et al. A reduction in Pten tumor suppressor activity promotes ErbB-2-induced mouse prostate adenocarcinoma formation through the activation of signaling cascades downstream of PDK1. Am J Pathol 174:2051-60 (2009). IHC-P, WB ; Human, Mouse . Read more (PubMed: 19443706) »
