Overview
- Product nameAnti-PDPK1 (phospho Y9) antibodySee all PDPK1 primary antibodies ...
- DescriptionRabbit polyclonal to PDPK1 (phospho Y9)
- Specificityab45375 detects a 65 kDa protein corresponding to the apparent molecular mass of PDPK1.
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide: RTTSQLYPDAVPIQS, corresponding to amino acids 3-16 of Human PDPK1
- Positive controlA431 cells treated with EGF, IGF-1, or pervanadate and adult mouse brain.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab45375 was cross-absorbed to phospho-tyrosine coupled to agarose, then affinity purified using phospho-PDPK1 (Tyr-9) peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab45375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa). |
| ELISA | ELISA: 1/2000. |
Target
- FunctionPhosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
- Tissue specificityAppears to be expressed ubiquitously.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain. - Post-translational
modificationsPhosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. - Cellular localizationCytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
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Database links
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 5170 Human
- Entrez Gene: 18607 Mouse
- Entrez Gene: 81745 Rat
- Omim: 605213 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: O15530 Human
- SwissProt: Q9Z2A0 Mouse
- SwissProt: O55173 Rat
- Unigene: 459691 Human
- Unigene: 10504 Mouse
- Unigene: 10905 Rat
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Target information above from: UniProt accession
O15530
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 3 Phosphoinositide Dependent Protein Kinase 1 antibody3-phosphoinositide-dependent protein kinase 1 antibodyhPDK 1 antibody
- hPDK1 antibodyMGC20087 antibodyMGC35290 antibodyOTTHUMP00000159109 antibodyOTTHUMP00000159110 antibodyOTTHUMP00000174525 antibodyPDK1 antibodyPDPK1 antibodyPDPK1_HUMAN antibodyPkB kinase antibodyPkB kinase like gene 1 antibodyPkB like 1 antibodyPRO0461 antibodyProtein kinase antibody
see all
References for Anti-PDPK1 (phospho Y9) antibody (ab45375)
ab45375 has not yet been referenced specifically in any publications.
