Anti-AS3 antibody [2030D32a] (ab60060)
Key features and details
- Mouse monoclonal [2030D32a] to AS3
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-AS3 antibody [2030D32a]
See all AS3 primary antibodies -
Description
Mouse monoclonal [2030D32a] to AS3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human AS3 (C terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab60060 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
2030D32a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at an assay dependent dilution. Detects a band of approximately 41 kDa (fragment), (predicted molecular weight: 165 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Plays a role in androgen-induced proliferative arrest in prostate cells. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the PDS5 family.
Contains 3 A.T hook DNA-binding domains.
Contains 1 HEAT repeat. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- AI646570 antibody
- Androgen induced proliferation inhibitor antibody
- Androgen-induced proliferation inhibitor antibody
see all
Images
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Anti-AS3 antibody [2030D32a] (ab60060) + immunising recombinant protein
Predicted band size: 165 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (0)
ab60060 has not yet been referenced specifically in any publications.