Anti-PDSS2 antibody [OTI1D12] (ab119768)
Key features and details
- Mouse monoclonal [OTI1D12] to PDSS2
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human, African green monkey, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-PDSS2 antibody [OTI1D12]
See all PDSS2 primary antibodies -
Description
Mouse monoclonal [OTI1D12] to PDSS2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human, African green monkey, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human PDSS2 aa 1-400. Produced in HEK-293T cells (NP_065114).
Database link: Q86YH6 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PDSS2 cDNA; HepG2 and COS7 cell extracts. IHC-P: Human kidney, lung carcinoma, endometrium adenocarcinoma and lymphoma tissues. Flow Cyt (Intra): HEK-293T cells transfected with PDSS2 overexpress plasmid.
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General notes
Clone OTI1D12 (formerly 1D12).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant -
Clonality
Monoclonal -
Clone number
OTI1D12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/100.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 44 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/100. |
Target
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Function
Supplies decaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone-10. -
Pathway
Cofactor biosynthesis; ubiquinone biosynthesis. -
Involvement in disease
Defects in PDSS2 are a cause of coenzyme Q10 deficiency (COQ10D) [MIM:607426]. Coenzyme Q10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy. -
Sequence similarities
Belongs to the FPP/GGPP synthase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 57107 Human
- Omim: 610564 Human
- SwissProt: Q86YH6 Human
- Unigene: 486095 Human
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Alternative names
- All-trans-decaprenyl-diphosphate synthase subunit 2 antibody
- bA59I9.3 antibody
- C6orf210 antibody
see all
Images
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Paraffin-embedded human kidney tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
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All lanes : Anti-PDSS2 antibody [OTI1D12] (ab119768) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY PDSS2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa -
All lanes : Anti-PDSS2 antibody [OTI1D12] (ab119768) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (canine kidney cell line) cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 44 kDa -
Paraffin-embedded human lung carcinoma tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human lymphoma tissue stained for PDSS2 using ab119768 at 1/150 dilution in immunohistochemical analysis.
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either PDSS2 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling pdss2 with ab119768 at 1/100 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab119768 has been referenced in 2 publications.
- Kanda M et al. Decreased expression of prenyl diphosphate synthase subunit 2 correlates with reduced survival of patients with gastric cancer. J Exp Clin Cancer Res 33:88 (2014). IHC-P ; Human . PubMed: 25330808
- Kanda M et al. Clinical utility of PDSS2 expression to stratify patients at risk for recurrence of hepatocellular carcinoma. Int J Oncol 45:2005-12 (2014). PubMed: 25189544