Anti-PDX1 antibody [OTI2A12] (ab84987)
Key features and details
- Mouse monoclonal [OTI2A12] to PDX1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PDX1 antibody [OTI2A12]
See all PDX1 primary antibodies -
Description
Mouse monoclonal [OTI2A12] to PDX1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-283 of human PDX1 expressed in E. coli.
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Positive control
- IHC-P: pancreas; carcinoma of pancreas WB: HEK293T cells transfected with PDX1; lysate from 293T cells transfected with PDX1 ICC/IF: HeLa cells transiently transfected by PDX1; HeLa cells
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General notes
The clone number has been updated from 2A12 to OTI2A12, both clone numbers name the same clone.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI2A12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/20000. Predicted molecular weight: 31 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/20000. Predicted molecular weight: 31 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. -
Tissue specificity
Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). -
Involvement in disease
Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Contains 1 homeobox DNA-binding domain. -
Domain
The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y). -
Post-translational
modificationsPhosphorylated by the SAPK2 pathway at high intracellular glucose concentration. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3651 Human
- Omim: 600733 Human
- SwissProt: P52945 Human
- Unigene: 32938 Human
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Alternative names
- Glucose sensitive factor antibody
- Glucose-sensitive factor antibody
- GSF antibody
see all
Images
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ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.
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ab84987, staining PDX1 in paraffin embedded human pancreas by Immunohistochemistry.
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Anti-PDX1 antibody [OTI2A12] (ab84987) at 1/20000 dilution + lysate from HEK293T cells transfected with human PDX1 expression vector
Predicted band size: 31 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033) -
All lanes : Anti-PDX1 antibody [OTI2A12] (ab84987)
Lane 1 : lysate from HEK293T cells transfected with an empty expression vector
Lane 2 : lysate from HEK293T cells transfected with a human PDX1 expression vector
Lysates/proteins at 5 µg per lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033) -
ab84987, staining PDX1 by Immunofluoresence in HeLa cells transiently transfected with a vector expressing PDX1.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab84987 has been referenced in 5 publications.
- Zhang X et al. Micro-RNA-124-5p promotes insulin producing cell differentiation through regulating transcriptional factor NKX6.1. Biochem Biophys Rep 30:101273 (2022). PubMed: 35592615
- Soltani A et al. Treatment of diabetic mice by microfluidic system-assisted transplantation of stem cells-derived insulin-producing cells transduced with miRNA. Life Sci 274:119338 (2021). PubMed: 33716064
- Jafarian A et al. The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9. PLoS One 10:e0128650 (2015). IF . PubMed: 26047014
- Allahverdi A et al. Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. Cell J 17:231-42 (2015). WB . PubMed: 26199902
- Jay CM et al. Assessment of intravenous pbi-shRNA PDX1 nanoparticle (OFHIRNA-PDX1) in yucatan swine. Cancer Gene Ther 20:683-9 (2013). PubMed: 24287722