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Question (45921) | PE / R-Phycoerythrin Conjugation Kit (ab102918)

Inquiry: I have a question, I know the product can be used to conjugate antibodies, but can it be used to conjugate proteins? Thank you.

Thank you for contacting us with your question.

Ab102918 has been developed to conjugate antibodies, however it targets available amine groups, so it can conjugate proteins and peptides provided there is at least one aminegroup (lysine) available.

For this reaction to be successful, the same restrictions do apply. The protein should be purified and in an amine free buffer (to prevent conjugation of buffer constituents), and the concentration should be at least 0.5 mg/mL. Depending on the protein size, the conjugation ratio can be very different than what is stated on the datasheet. For instance, many small peptides can conjugate to one molecule of PE, whereas we would expect an antibody to PE ratio of 1:1.

I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you and I'll be happy to help.

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