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I need to stain surface FREM2 protein on MCF7 cell line for following application in flow cytometry experiment. I found Abcam Anti-FREM2 antibody (ab75803) and EasyLink R-Phycoerythrin Conjugation Kit (3 x 100µg R-PE) (ab102918) – are these products suitable for flow cytometry? There’s 1% BSA in FREM2 antibody’s solution – will BSA interfere with R-PE labeling because of aminogroups?
Asked on Dec 10 2012
Thank you for contacting us. We have not tested ab75803 in Flow Cytometry and do not have any data regarding suitability in this application. You would have to purify the antibody before conjugating it to remove the BSA or the BSA may be conjugated.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Abcam Scientific Support
Answered on Dec 11 2012