Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-PEN2 antibody - Carboxyterminal end
See all PEN2 products (7) ...
Description
Rabbit polyclonal to PEN2 - Carboxyterminal end
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Human) of 13 amino acids from near the C-terminus of PEN2
Positive control
K562 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Notch Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF: Use a concentration of 20 µg/ml.
Target
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
Sequence similarities
Belongs to the PEN-2 family.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Target information above from: UniProt accessionQ9NZ42
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gamma secretase subunit PEN 2 antibody
- Gamma Secretase Subunit PEN2 antibody
- Gamma-secretase subunit PEN-2 antibody
see all
Database links
- Entrez Gene: 55851 Human
- Entrez Gene: 66340 Mouse
- Entrez Gene: 81751 Rat
- Entrez Gene: 292788 Rat
- Omim: 607632 Human
- SwissProt: Q9NZ42 Human
- SwissProt: Q9CQR7 Mouse
- SwissProt: O88777 Rat
see all
Anti-PEN2 antibody - Carboxyterminal end images:
Western blot - PEN2 antibody (ab62514)
Lane 1 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 0.5 µg/ml
Lane 2 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 1 µg/ml
Lane 3 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 12 kDa
Observed band size : 12 kDa
Immunocytochemistry/ Immunofluorescence - PEN2 antibody (ab62514)
Immunofluorescence of K562 cells using ab62514 at 20 µg/ml.
References for Anti-PEN2 antibody - Carboxyterminal end (ab62514)
This product has been referenced in:
- Area-Gomez E et al. Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria. Am J Pathol 175:1810-6 (2009). WB; Mouse.Read more (PubMed: 19834068) »
See 1 publication for this product
Publishing research using ab62514? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"