![Western blot - PEP1 antibody [18C8BC7AD10] (ab113690) image](#PEP1-Primary-antibodies-ab113690-2.jpg)
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Product name
Anti-PEP1 antibody [18C8BC7AD10]
Description
Mouse monoclonal [18C8BC7AD10] to PEP1
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant full length protein
Positive control
Yeast lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Clonality
Monoclonal
Clone number
18C8BC7AD10
Isotype
IgG2a
Light chain type
kappa
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> ER Proteins
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Western blot - PEP1 antibody [18C8BC7AD10] (ab113690)
![Western blot - PEP1 antibody [18C8BC7AD10] (ab113690) image](/ps/datasheet/images/113/ab113690/PEP1-Primary-antibodies-ab113690-2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab113690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
Target
Function
Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.
Sequence similarities
Belongs to the VPS10-related sortilin family.
Contains 10 BNR repeats.
Domain
The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.
Cellular localization
Golgi apparatus > trans-Golgi network membrane. Prevacuolar compartment membrane. Cycles between the Golgi apparatus and the prevacuolar compartments.
Target information above from: UniProt accessionP32319
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Carboxypeptidase Y receptor antibody
- Carboxypeptidase Y-deficient protein 1 antibody
- CPY receptor antibody
see all
Database links
- Entrez Gene: 852264 Saccharomyces cerevisiae
- SwissProt: P32319 Saccharomyces cerevisiae
Anti-PEP1 antibody [18C8BC7AD10] images:
Western blot - PEP1 antibody [18C8BC7AD10] (ab113690)
Anti-PEP1 antibody [18C8BC7AD10] (ab113690) + Yeast whole cell lysate at 15 µg
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 177 kDa
References for Anti-PEP1 antibody [18C8BC7AD10] (ab113690)
ab113690 has not yet been referenced specifically in any publications.
Publishing research using ab113690? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"