Anti-PER3 antibody [4B9D7] (ab201940)
Key features and details
- Mouse monoclonal [4B9D7] to PER3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PER3 antibody [4B9D7]
See all PER3 primary antibodies -
Description
Mouse monoclonal [4B9D7] to PER3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PER3 aa 700-1000. (Purified from E.coli).
Database link: P56645 -
Positive control
- Human PER3 (aa723-954) recombinant protein; PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate; HeLa cells; Human ovarian cancer and rectum cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
4B9D7 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 132 kDa.
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ICC/IF |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 132 kDa. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme. -
Sequence similarities
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylation appears to require association with PER1 and translocation to the nucleus.
Ubiquitinated. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS. - Information by UniProt
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Database links
- Entrez Gene: 8863 Human
- Omim: 603427 Human
- SwissProt: P56645 Human
- Unigene: 162200 Human
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Alternative names
- 2810049O06Rik antibody
- Cell growth inhibiting gene 13 protein antibody
- Cell growth-inhibiting gene 13 protein antibody
see all
Images
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Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution + Human PER3 (aa723-954) recombinant protein
Predicted band size: 132 kDaExpected MWt is 50.7 kDa.
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All lanes : Anti-PER3 antibody [4B9D7] (ab201940) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PER3 (aa723-954)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 132 kDa(Expected MW is 50.7 kDa)
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Immunofluorescent analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow cytometric analysis of HeLa cells labeling PER3 with ab201940 at 1/200 dilution (green) compared to a negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling PER3 with ab201940 at 1/200 dilution, with DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab201940 has been referenced in 1 publication.
- Knarr M et al. miR-181a modulates circadian rhythm in immortalized bone marrow and adipose derived stromal cells and promotes differentiation through the regulation of PER3. Sci Rep 9:307 (2019). PubMed: 30670712