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Anti-PEX14 antibody (ab76862) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 41 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Detection limit for ab76862 is approximately 3ng/ml as a capture antibody.
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