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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Overview
Product name
Anti-PEX14 antibody
See all PEX14 products (5) ...
Description
Mouse monoclonal to PEX14
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human
Immunogen
Recombinant fragment: LGPQEEGEGV VDVKGQVRME VQGEEEKRED KEDEEDEEDD DVSHVDEEDC LGVQREDRRG GDGQINEQVE KLRRPEGASN ESE, corresponding to amino acids 293-375 of human PEX14 (NP_004556) with a 26 kDa tag.
LGPQEEGEGV VDVKGQVRME VQGEEEKRED KEDEEDEEDD DVSHVDEEDC LGVQREDRRG GDGQINEQVE KLRRPEGASN ESE
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 41 kDa.(This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. )
ELISA: Use at an assay dependent dilution. (Detection limit ~3ng/ml when used as a capture antibody.)
Target
Function
Component of the peroxisomal translocation machinery with PEX13 and PEX17. Interacts with both the PTS1 and PTS2 receptors. Binds directly to PEX17.
Involvement in disease
Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) [MIM:601791]. PBD-CGK is a peroxisomal disorder arising from a failure of protein import into the peroxisomal membrane or matrix. The peroxisome biogenesis disorders (PBD group) are genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Include disorders are: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum (PBD-ZSS).
Defects in PEX14 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life.
Sequence similarities
Belongs to the peroxin-14 family.
Cellular localization
Peroxisome membrane.
Target information above from: UniProt accessionO75381
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- NAPP2 antibody
- NF E2 associated polypeptide 2 antibody
- Peroxin 14 antibody
see all
Database links
- Entrez Gene: 5195 Human
- Omim: 601791 Human
- SwissProt: O75381 Human
- Unigene: 149983 Human
Anti-PEX14 antibody images:
Western blot - PEX14 antibody (ab76862)
Anti-PEX14 antibody (ab76862) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
Predicted band size : 41 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Sandwich ELISA - PEX14 antibody (ab76862)
Detection limit for ab76862 is approximately 3ng/ml as a capture antibody.
References for Anti-PEX14 antibody (ab76862)
ab76862 has not yet been referenced specifically in any publications.
Publishing research using ab76862? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"