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Overview

  • Product namePF4 Mouse ELISA KitSee all PF4 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 6 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 73.99 67% - 83%
    Serum 118.2 104% - 127%
    Plasma 109.3 92% - 129%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100735 Mouse PF4 (platelet factor 4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse PF4 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications    Binds non-covalently to a proteoglycan molecule.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P02776 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        C X C motif chemokine 4C-X-C motif chemokine 4Chemokine (C X C motif) ligand 4
        Chemokine (CXC motif) ligand 4CXCL 4CXCL4IroplactMGC138298 Oncostatin AOncostatin-AOncostatinA PF 4PF-4PF4Pf4aPlatelet factor 4PLF4_HUMANRATPF4ASCYB 4SCYB4short formSmall inducible cytokine subfamily member 4
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100735 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      PF4 Mouse ELISA Kit images

      • ELISA - PF4 Mouse ELISA Kit (ab100735)
        ELISA - PF4 Mouse ELISA Kit (ab100735)

      Protocols

      References for PF4 Mouse ELISA Kit (ab100735)

      ab100735 has not yet been referenced specifically in any publications.

      Publishing research using ab100735 ? Please let us know so that we can cite the reference in this datasheet

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