Overview

  • Product nameAnti-PFD1145c antibodySee all PFD1145c primary antibodies ...
  • Description
    Mouse polyclonal to PFD1145c
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Plasmodium falciparum
  • Immunogen

    Recombinant fusion protein containing a His tag:

    HPYDINNKNDDSYRYDISEE

    , corresponding to amino acids 240-259 of Plasmodium falciparum PFD1145c.

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab52699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancePFD1145c is a putative uncharacterized protein from Plasmodium falciparum>.
    • Database links
      • Alternative names
        • Hypothetical protein PFD1145c antibody

      Anti-PFD1145c antibody images

      • All lanes : Anti-PFD1145c antibody (ab52699) at 1/1000 dilution

        Lane 1 : ~20µg of a total protein extract from E coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
        Lane 2 : ~20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag)

        Secondary
        Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      References for Anti-PFD1145c antibody (ab52699)

      ab52699 has not yet been referenced specifically in any publications.

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