- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PFKFB1 antibody - N-terminal
See all PFKFB1 primary antibodies -
DescriptionRabbit polyclonal to PFKFB1 - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide from the N terminal region of human PFKFB1 conjugated to KLH
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Positive control
- Jurkat cell line lysate and human hepatocarcinoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesab71626 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab71626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1000. | |
| WB | 1/100 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). | |
| IHC-P | 1/50 - 1/100. |
Target
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FunctionSynthesis and degradation of fructose 2,6-bisphosphate.
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Tissue specificityLiver.
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Sequence similaritiesIn the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
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Database links
- Entrez Gene: 5207 Human
- Entrez Gene: 5208 Human
- Entrez Gene: 5209 Human
- Entrez Gene: 5210 Human
- Entrez Gene: 18639 Mouse
- Entrez Gene: 18640 Mouse
- Entrez Gene: 270198 Mouse
- Omim: 311790 Human
see all -
Alternative names
- 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 1 antibody
- 6-bisphosphatase antibody
- 6-P2ase 1 antibody
see all
Images
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Western blot - PFKFB1 antibody - N-terminal (ab71626)Anti-PFKFB1 antibody - N-terminal (ab71626) at 1/240 dilution + Jurkat cell line lysate at 35 µg
Predicted band size: 55 kDa
Observed band size: 60 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PFKFB1 antibody - N-terminal (ab71626)ab71626 at 1/50 dilution, staining PFKFB1 in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
References
ab71626 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"