Anti-PFKP antibody (ab186132)
Key features and details
- Rabbit polyclonal to PFKP
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PFKP antibody
See all PFKP primary antibodies -
Description
Rabbit polyclonal to PFKP -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human PFKP aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_002618.1).
Database link: Q01813 -
Positive control
- HeLa, 293T, Jurkat and NIH 3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab186132 was affinity purified using an epitope specific to PFKP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 86 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 86 kDa. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. -
Sequence similarities
Belongs to the phosphofructokinase family. Two domains subfamily. - Information by UniProt
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Database links
- Entrez Gene: 450266 Chimpanzee
- Entrez Gene: 5214 Human
- Entrez Gene: 56421 Mouse
- Entrez Gene: 100173619 Orangutan
- Entrez Gene: 722330 Rhesus monkey
- Omim: 171840 Human
- SwissProt: Q01813 Human
- SwissProt: Q9WUA3 Mouse
see all -
Alternative names
- 1200015H23Rik antibody
- 6 phosphofructokinase type C antibody
- 6 phosphofructokinase, platelet type antibody
see all
Images
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All lanes : Anti-PFKP antibody (ab186132) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes -
Detection of PFKP in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186132 at 6 µg/reaction for IP. For WB detection, ab186132 was used at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186132 has been referenced in 1 publication.
- Wang H et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature 546:426-430 (2017). PubMed: 28607489